ArticlePeerJ2020
Measuring recognition memory in zebrafish larvae: issues and limitations.
Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- zAcademy: an open-source programmable conditioning arena reveals sex- and genotype-dependent avoidance learning in adult zebrafish.bioRxiv : the preprint server for biology · 2026Article
- Ontogeny of Rat Recognition Memory, Exploratory Activity and Anxiety Measured by Novel Object Recognition Test and Open Field Test.Annals of neurosciences · 2026Article
- A potential role for BDNF in recognition behavior revealed through knockout in zebrafish.Frontiers in behavioral neuroscience · 2026Article
- Environmental enrichment influences the relationship between lateralization and neophobia in zebrafish larvae.Animal cognition · 2025Article
- Zebrafish as an Integrative Model for Central Nervous System Research: Current Advances and Translational Perspectives.Life (Basel, Switzerland) · 2025Review
- Habituation with apparatus and group testing improves assessment of fish preferences.Journal of fish biology · 2025Article
- Butylated Hydroxyanisole (BHA) Disrupts Brain Signalling in Embryo-Larval Stage of Zebrafish Leading to Attention Deficit Hyperactivity Disorder (ADHD).Journal of xenobiotics · 2025Article
- Assessment of Replicability and Efforts to Refine an Operant Conditioning Procedure for Larval Zebrafish.Animals : an open access journal from MDPI · 2024Article
- Exploring the Importance of Environmental Complexity for Newly Hatched Zebrafish.Animals : an open access journal from MDPI · 2024Article
- Embryonic ethanol exposure and optogenetic activation of hypocretin neurons stimulate similar behaviors early in life associated with later alcohol consumption.Scientific reports · 2024Article
- Novel object recognition in Octopus maya.Animal cognition · 2023Article
- Fentanyl Induces Novel Conditioned Place Preference in Adult Zebrafish, Disrupts Neurotransmitter Homeostasis, and Triggers Behavioral Changes.International journal of environmental research and public health · 2022Article
- Article
- Environmental enrichment decreases anxiety-like behavior in zebrafish larvae.Developmental psychobiology · 2022Article
- Visual discrimination and amodal completion in zebrafish.PloS one · 2022Article
- Baicalein 5,6-Dimethyl Ether Prevents Memory Deficits in the Scopolamine Zebrafish Model by Regulating Cholinergic and Antioxidant Systems.Plants (Basel, Switzerland) · 2021Article
- Automated Operant Conditioning Devices for Fish. Do They Work?Animals : an open access journal from MDPI · 2021Article
- About Welfare and Stress in the Early Stages of Fish.Frontiers in veterinary science · 2021Article
- Environmental and Molecular Modulation of Motor Individuality in Larval Zebrafish.Frontiers in behavioral neuroscience · 2021Article
- Development of Open-Field Behaviour in the Medaka,Biology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recognition memory is the capacity to recognize previously encountered objects, events or places. This ability is crucial for many fitness-related activities, and it appears very early in the development of several species. In the laboratory, recognition memory is most often investigated using the novel object recognition test (NORt), which exploits the tendency of most vertebrates to explore novel objects over familiar ones. Despite that the use of larval zebrafish is rapidly increasing in research on brain, cognition and neuropathologies, it is unknown whether larvae possess recognition memory and whether the NORt can be used to assess it. Here, we tested a NOR procedure in zebrafish larvae of 7-, 14- and 21-days post-fertilization (dpf) to investigate when recognition memory first appears during ontogeny. Overall, we found that larvae explored a novel stimulus longer than a familiar one. This response was fully significant only for 14-dpf larvae. A control experiment evidenced that larvae become neophobic at 21-dpf, which may explain the poor performance at this age. The preference for the novel stimulus was also affected by the type of stimulus, being significant with tri-dimensional objects varying in shape and bi-dimensional geometrical figures but not with objects differing in colour. Further analyses suggest that lack of effect for objects with different colours was due to spontaneous preference for one colour. This study highlights the presence of recognition memory in zebrafish larvae but also revealed non-cognitive factors that may hinder the application of NORt paradigms in the early developmental stages of zebrafish.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.