ArticleAmerican journal of cancer research2020
USP18 deubiquitinates and stabilizes Twist1 to promote epithelial-mesenchymal transition in glioblastoma cells.
Article in American journal of cancer research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- USP18 deubiquitinates and stabilizes SOX9 to promote the stemness and malignant progression of glioblastoma.Cell death discovery · 2025Article
- Deubiquitinase USP18 mediates cell migration, apoptosis and ferroptosis in lung adenocarcinoma by depending on POU4F1/PRKAA2 axis.BMC cancer · 2025Article
- Role of Ubiquitin-regulated EMT in Cancer Metastasis and Chemoresistance.International journal of biological sciences · 2025Review
- PL-hMSC and CH-hMSC derived soluble factors inhibit proliferation but improve hGBM cell migration by activating TGF-β and inhibiting Wnt signaling.Bioscience reports · 2024Article
- Bulk and single cells transcriptomes with experimental validation identify USP18 as a novel glioma prognosis and proliferation indicator.Experimental and therapeutic medicine · 2024Article
- Review
- Ubiquitin-specific protease 5 promotes bladder cancer progression through stabilizing Twist1.Oncogene · 2024Article
- Friend or foe? Reciprocal regulation between E3 ubiquitin ligases and deubiquitinases.Biochemical Society transactions · 2024Review
- Loss of ubiquitin-specific peptidase 18 destabilizes 14-3-3ζ protein and represses lung cancer metastasis.Cancer biology & therapy · 2022Article
- The anterior gradient homologue 2 (AGR2) co-localises with the glucose-regulated protein 78 (GRP78) in cancer stem cells, and is critical for the survival and drug resistance of recurrent glioblastoma: in situ and in vitro analyses.Cancer cell international · 2022Article
- The diverse repertoire of ISG15: more intricate than initially thought.Experimental & molecular medicine · 2022Review
- Identification of ubiquitin-specific protease 32 as an oncogene in glioblastoma and the underlying mechanisms.Scientific reports · 2022Article
- Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1.BMC cancer · 2022Article
- Development of a novel glycolysis-related genes signature for isocitrate dehydrogenase 1-associated glioblastoma multiforme.Frontiers in immunology · 2022Article
- CircCRIM1 Ameliorates Endothelial Cell Angiogenesis in Aging through the miR-455-3p/Twist1/VEGFR2 Signaling Axis.Oxidative medicine and cellular longevity · 2022Article
- Deletion of miR-15a inhibited glioma development via targeting Smad7 and inhibiting EMT pathway.Aging · 2021Article
- USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner.Oncology letters · 2021Article
- PRL1 Promotes Glioblastoma Invasion and TumorigenesisFrontiers in oncology · 2021Article
- Emerging Roles of USP18: From Biology to Pathophysiology.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aberrant activation of epithelial-mesenchymal transition (EMT) pathway drives the invasion and migration of multiple cancers including glioblastoma (GBM). Clinical interventions focused on inhibiting EMT are of increasing interest in the treatment of GBM. In the present study, we discovered that glioma tissues and cells, especially GBMs show significantly up-modulated ubiquitin-specific protease 18 (USP18) expression. Functionally, decreased USP18 expression attenuated GBM cell invasion and migration through repressing EMT. Moreover, a critical EMT-inducing transcription factor Twist1 that activates EMT, was identified as a downstream target of USP18. Mechanistically, USP18 interacts with Twist1, removes its ubiquitination off, and subsequently stabilizes it. Short hairpin RNA-mediated downregulation of USP18 accelerates Twist1 degradation, resulting in the inhibition of GBM cell invasion and migration in vitro and in a nude mouse model. Importantly, reconstituted expression of Twist1 almost completely rescues the inhibitory effect of USP18 depletion on GBM cell invasion, migration and tumor formation. Clinically, the expression levels of USP18 and Twist1 are positively relevant in GBM specimens, and high expression of USP18 correlates with patient's poor outcome. Finally, our findings unveil the crucial role of USP18 on GBM malignancy. Targeting USP18-Twist1 regulatory axis may open a novel avenue for GBM treatment.
Indexed as
Identifiers
32368392PMC7191102W3023791973What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.