Evidence map›Paper›PMID 32367481›Full record

ArticleStem cell reviews and reports2020

Role of Chromatin Remodeling Genes and TETs in the Development of Human Midbrain Dopaminergic Neurons.

Li Xiang, Guobin Huang, Wenjing Shu, Chunli Gong, Nan Cao, Rui Chen, Jixi Li, Hao Lu, Gaofeng Jiang

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In one paragraph

Article in Stem cell reviews and reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Li XiangCenter for Translational Medicine, Tianyou Hospital, Wuhan University of Science and Technology, No.9, Tujialing, Wuchang District, Wuhan, 430064, Hubei, China.ORCID 0000-0002-3941-9330
Guobin HuangMinistry of Education (MOE) Key Laboratory of Gene Function and Regulation, School of Life Sciences, State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, 510006, Guangdong, China.ORCID 0000-0002-8985-9238
Wenjing ShuCenter for Translational Medicine, Tianyou Hospital, Wuhan University of Science and Technology, No.9, Tujialing, Wuchang District, Wuhan, 430064, Hubei, China.ORCID 0000-0002-8970-8499
Chunli GongLevi Regenerative Medicine Technologies, Zhuhai, 519085, Guangdong, China.ORCID 0000-0001-8045-0121
Nan CaoCenter for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, 510080, Guangdong, China.
Rui ChenDepartment of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, 100069, China.
Jixi LiState Key Laboratory of Genetic Engineering, School of Life Sciences and Huashan Hospital, Collaborative Innovation Center of Genetics and Development, Fudan University, Shanghai, 200438, China.
Hao LuLevi Regenerative Medicine Technologies, Zhuhai, 519085, Guangdong, China.ORCID 0000-0002-7742-0316
Gaofeng JiangCenter for Translational Medicine, Tianyou Hospital, Wuhan University of Science and Technology, No.9, Tujialing, Wuchang District, Wuhan, 430064, Hubei, China. jianggaofeng@wust.edu.cn.
Sun Yat-sen University · CNWuhan University of Science and Technology · CNCapital Medical University · CNFudan University · CNWuchang University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding epigenetic regulation in the differentiation and maturation of dopaminergic neurons is critical to improve and develop new medications for Parkinson's disease (PD). To explore the role of ten-eleven translocation (TETs) family of dioxygenases and chromatin remodeling genes in the development of human midbrain dopaminergic (mDA) neurons, we globally analyze the epigenetic regulation of gene expression in human induced pluripotent stem cells (iPSCs) and iPSCs-derived mDA neurons. During the conversion of iPSCs into neuronal lineages of dopaminergic progenitors and mDA neurons, the expression patterns of epigenetic genes in multiple sets alter significantly. Vitamin C, an activator of TET enzymes, increases hydroxymethylcytosine (5hmC) level along with a higher yield of mDA neurons. Additionally, vitamin C treatment elevates gene expressions of TET2/3 and vitamin C transporters. Importantly, functional arrays indicate that vitamin C can promote neuronal maturation, synaptic activity, and dopamine release. Collectively, our study demonstrates that chromatin remodeling genes and the TET-5hmC pathway, which is regulated by vitamin C, are critical for the vital developmental stages of human mDA neurons.

Indexed as

5-MethylcytosineAdultAgedAscorbic AcidCell DifferentiationCell LineCell LineageChromatin Assembly and DisassemblyDioxygenasesDNA-Binding ProteinsDopamineDopaminergic NeuronsEpigenesis, GeneticHumansInduced Pluripotent Stem CellsMesencephalon5-hydroxymethylcytosine5-MethylcytosineAscorbic AcidDioxygenasesDNA-Binding ProteinsDopamineProto-Oncogene ProteinsTET2 protein, humanTET3 protein, human5hmCDopaminergic neuronEpigeneticsTET enzymesVitamin C

Identifiers

PMID32367481
OpenAlexW3022713856

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.