ArticleMolecular genetics and genomics : MGG2020
Heat-shock-induced tyrosinase gene ablation with CRISPR in zebrafish.
Article in Molecular genetics and genomics : MGG, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Comparative Transcriptome and Gene-Editing Analyses Reveal the Molecular Mechanism of Albinism in the Taiwanese Loach.Marine biotechnology (New York, N.Y.) · 2026Article
- Novel drug-inducible CRISPRa/i systems for rapid and reversible manipulation of gene transcription.Cellular and molecular life sciences : CMLS · 2025Article
- Beyond drug discovery: Exploring the physiological and methodological dimensions of zebrafish in diabetes research.Experimental physiology · 2024Review
- Review
- Article
- Reactivation of the Neurogenic Niche in the Adult Zebrafish Statoacoustic Ganglion Following a Mechanical Lesion.Frontiers in cell and developmental biology · 2022Article
- Rapid generation of maternal mutants via oocyte transgenic expression of CRISPR-Cas9 and sgRNAs in zebrafish.Science advances · 2021Article
- Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish.Nature communications · 2021Article
- Zebrafish for Personalized Regenerative Medicine; A More Predictive Humanized Model of Endocrine Disease.Frontiers in endocrinology · 2020Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Tyrosinase (TYR) converts L-tyrosine into 3,4-dihydroxyphenylalanine (L-DOPA) and L-DOPA into L-dopaquinone, which can produce melanin pigment. The abrogation of the functional activity of TYR can result in albino skin and eye diseases because of a deficiency in melanin pigment production. In this study, we developed and characterized an inducible knockout TYR platform comprising the heat-inducible heat-shock-promoter-70-driving CRISPR/Cas9 system and a zU6-promoter-driving tyr single guide RNA (sgRNA) system to investigate the temporal expression of TYR genes. To overcome the difficulty of identifying zebrafish germline integrations and facilitate the observation of Cas9 expression, heart-specific cmlc2:enhanced green fluorescent protein (EGFP; used to confirm tyr sgRNA expression) and two selectable markers (P2A-mCherry and internal ribosomal entry site-EGFP) were applied in our system. Heat shock treatment administered to Cas9 transgenic embryos induced mCherry or EGFP fluorescence expression throughout the embryos' bodies, and Cas9 protein was detected 1 h after heat shock treatment. Mutations were created by direct injection and line crossing, which led to mosaic and complete depigmentation phenotypes in approximately 50% and 100% of the embryos, respectively. Using our system, conditional TYR knockout in zebrafish was achieved efficiently and simply.
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Registered trials
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