ArticleScientific reports2020
Invasion of Human Retinal Pigment Epithelial Cells by Porphyromonas gingivalis leading to Vacuolar/Cytosolic localization and Autophagy dysfunction In-Vitro.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Exploring Endotoxemia in Age-Related Macular Degeneration, Glaucoma, and Diabetic Retinopathy.Investigative ophthalmology & visual science · 2026Review
- Systemic inflammation at the oral-ocular interface: a 3P medicine perspective on the relationship between periodontitis and eye diseases.The EPMA journal · 2025Article
- Is periodontitis a potential risk factor of rhegmatogenous retinal detachment? A population-based retrospective cohort study of Taiwan.Journal of dental sciences · 2025Article
- Hallmarks of aging in age-related macular degeneration and age-related neurological disorders: novel insights into common mechanisms and clinical relevance.Eye (London, England) · 2025Review
- Macrophage-Hosted Porphyromonas gingivalis Is a Risk Factor for Cataract Development.Investigative ophthalmology & visual science · 2025Article
- A perspective from the National Eye Institute Extracellular Vesicle Workshop: Gaps, needs, and opportunities for studies of extracellular vesicles in vision research.Journal of extracellular vesicles · 2024Review
- Microbiome as an endocrine organ and its relationship with eye diseases: Effective factors and new targeted approaches.World journal of gastrointestinal pathophysiology · 2024Review
- Opportunistic pathogeniScience · 2024Article
- Increased caveolin 1 by human antigen R exacerbatesCytoJournal · 2024Article
- Do Oral Pathogens Inhabit the Eye and Play a Role in Ocular Diseases?Journal of clinical medicine · 2022Review
- Editorial: Xenophagy: Its role in pathogen infections.Frontiers in cellular and infection microbiology · 2022Article
- Interaction Between Autophagy andFrontiers in cellular and infection microbiology · 2022Review
- The Antimicrobial Susceptibility ofAntibiotics (Basel, Switzerland) · 2021Article
- Exacerbation of AMD Phenotype in Lasered CNV Murine Model by Dysbiotic Oral Pathogens.Antioxidants (Basel, Switzerland) · 2021Article
- Exposure to Porphyromonas gingivalis and Modifiable Risk Factors Modulate Risk for Early Diabetic Retinopathy.Translational vision science & technology · 2021Article
- IntracellularFrontiers in cellular and infection microbiology · 2020Article
- Could Infectious Agents Play a Role in the Onset of Age-related Macular Degeneration? A Scoping Review.Ophthalmology scienceReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent epidemiological studies link Periodontal disease(PD) to age-related macular degeneration (AMD). We documented earlier that Porphyromonas gingivalis(Pg), keystone oral-pathobiont, causative of PD, efficiently invades human gingival epithelial and blood-dendritic cells. Here, we investigated the ability of dysbiotic Pg-strains to invade human-retinal pigment epithelial cells(ARPE-19), their survival, intracellular localization, and the pathological effects, as dysfunction of RPEs leads to AMD. We show that live, but not heat-killed Pg-strains adhere to and invade ARPEs. This involves early adhesion to ARPE cell membrane, internalization and localization of Pg within single-membrane vacuoles or cytosol, with some nuclear localization apparent. No degradation of Pg or localization inside double-membrane autophagosomes was evident, with dividing Pg suggesting a metabolically active state during invasion. We found significant downregulation of autophagy-related genes particularly, autophagosome complex. Antibiotic protection-based recovery assay further confirmed distinct processes of adhesion, invasion and amplification of Pg within ARPE cells. This is the first study to demonstrate invasion of human-RPEs, begin to characterize intracellular localization and survival of Pg within these cells. Collectively, invasion of RPE by Pg and its prolonged survival by autophagy evasion within these cells suggest a strong rationale for studying the link between oral infection and AMD pathogenesis in individuals with periodontitis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.