ArticleBiomolecules2020
Differentiation of Murine C2C12 Myoblasts Strongly Reduces the Effects of Myostatin on Intracellular Signaling.
Article in Biomolecules, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- Engineering drug-responsive replication machinery for precise control of self-amplifying RNA.Nature biomedical engineering · 2026Article
- DUSP29 does not regulate melanoma-myoblast interactions in a skeletal muscle co-culture model.Scientific reports · 2026Article
- Myoblast and fibroblast derived small extracellular vesicles differentially affect myoblast migration dynamics.Journal of muscle research and cell motility · 2025Article
- Engineering gene expression dynamics via self-amplifying RNA with drug-responsive non-structural proteins.bioRxiv : the preprint server for biology · 2025Article
- The presence of differentiated C2C12 muscle cells enhances toxin production and growth byVirulence · 2024Article
- Interaction of the C2C12 myotube contractions and glucose availability on transcriptome and extracellular vesicle microRNAs.American journal of physiology. Cell physiology · 2024Article
- Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells.Molecular biology reports · 2023Article
- Nanomedicine for Treating Muscle Dystrophies: Opportunities, Challenges, and Future Perspectives.International journal of molecular sciences · 2022Review
- Applying exercise-mimetic engineered skeletal muscle model to interrogate the adaptive response of irisin to mechanical force.iScience · 2022Article
- Extracellular vesicles derived from tumour cells as a trigger of energy crisis in the skeletal muscle.Journal of cachexia, sarcopenia and muscle · 2022Article
- Systems for Muscle Cell Differentiation: From Bioengineering to Future Food.Micromachines · 2021Review
- Article
- Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation.American journal of physiology. Endocrinology and metabolism · 2021Article
- Review
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alongside in vivo models, a simpler and more mechanistic approach is required to study the effects of myostatin on skeletal muscle because myostatin is an important negative regulator of muscle size. In this study, myostatin was administered to murine (C2C12) and human (CHQ) myoblasts and myotubes. Canonical and noncanonical signaling downstream to myostatin, related ligands, and their receptor were analyzed. The effects of tumorkines were analyzed after coculture of C2C12 and colon cancer-C26 cells. The effects of myostatin on canonical and noncanonical signaling were strongly reduced in C2C12 cells after differentiation. This may be explained by increased follistatin, an endogenous blocker of myostatin and altered expression of activin receptor ligands. In contrast, CHQ cells were equally responsive to myostatin, and follistatin remained unaltered. Both myostatin administration and the coculture stimulated pathways associated with inflammation, especially in C2C12 cells. In conclusion, the effects of myostatin on intracellular signaling may be cell line- or organism-specific, and C2C12 myotubes seem to be a nonoptimal in vitro model for investigating the effects of myostatin on canonical and noncanonical signaling in skeletal muscle. This may be due to altered expression of activin receptor ligands and their regulators during muscle cell differentiation.
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