Evidence map›Paper›PMID 32365353›Full record

ArticleNature2020

Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform.

Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Philip V'kovski, Hanspeter Stalder, Jasmine Portmann, Jenna Kelly, Silvio Steiner, Melle Holwerda, Annika Kratzel and 18 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Nature, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 312 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
312citing papers in PubMed, 2 pooled it
10.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

312 citing papers in PubMed, 2 syntheses or guidelines pooled it, 519 citations in OpenAlex.

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  8. Evaluation of a proposed link between the SARS-CoV-2 furin cleavage site and mouse-adapted MERS-coronavirus MA30.Proceedings of the National Academy of Sciences of the United States of America · 2026
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252 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

28 authors at 3 institutions in 4 countries.

Tran Thi Nhu Thao *Institute of Virology and Immunology (IVI), Bern, Switzerland.
Fabien Labroussaa *Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.ORCID http://orcid.org/0000-0002-8712-3024
Nadine Ebert *Institute of Virology and Immunology (IVI), Bern, Switzerland.
Philip V'kovskiInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Hanspeter StalderInstitute of Virology and Immunology (IVI), Bern, Switzerland.ORCID http://orcid.org/0000-0003-0763-6469
Jasmine PortmannInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Jenna KellyInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Silvio SteinerInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Melle HolwerdaInstitute of Virology and Immunology (IVI), Bern, Switzerland.ORCID http://orcid.org/0000-0002-9814-7793
Annika KratzelInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Mitra GultomInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Kimberly SchmiedInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Laura LaloliInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Linda HüsserInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Manon WiderInsitute for Infectious Diseases, University of Bern, Bern, Switzerland.
Stephanie PfaenderInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Dagny HirtInstitute of Virology and Immunology (IVI), Bern, Switzerland.
Valentina CippàDepartment of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Silvia Crespo-PomarDepartment of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Simon SchröderInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Doreen MuthInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Daniela NiemeyerInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.ORCID http://orcid.org/0000-0002-1897-6365
Victor M CormanInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Marcel A MüllerInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
Christian DrostenInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.ORCID http://orcid.org/0000-0001-7923-0519
Ronald DijkmanInstitute of Virology and Immunology (IVI), Bern, Switzerland.ORCID http://orcid.org/0000-0003-0320-2743
Joerg JoresDepartment of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. joerg.jores@vetsuisse.unibe.ch.ORCID http://orcid.org/0000-0003-3790-5746
Volker ThielInstitute of Virology and Immunology (IVI), Bern, Switzerland. volker.thiel@vetsuisse.unibe.ch.ORCID http://orcid.org/0000-0002-5783-0887
University of Bern · CHHumboldt-Universität zu Berlin · DESechenov University · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reverse genetics has been an indispensable tool to gain insights into viral pathogenesis and vaccine development. The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate in Escherichia coli owing to the size and occasional instability of the genome

Indexed as

AnimalsBetacoronavirusChinaChlorocebus aethiopsChromosomes, Artificial, YeastCloning, MolecularCoronavirus InfectionsCOVID-19DNA-Directed RNA PolymerasesEvolution, MolecularGenome, ViralGenomicsHumansMutationPandemicsPneumonia, Viralbacteriophage T7 RNA polymeraseDNA-Directed RNA PolymerasesViral Proteins

Identifiers

PMID32365353
OpenAlexW3021391231

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.