ArticlePediatric research2021
Rho/SMAD/mTOR triple inhibition enables long-term expansion of human neonatal tracheal aspirate-derived airway basal cell-like cells.
Article in Pediatric research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- An immune-poised state of human bronchial epithelial cells mediates defense against RSV in adults.Nature communications · 2026Article
- Mammalian aging involves genome-wide splicing degeneration leading to functional decline.bioRxiv : the preprint server for biology · 2026Article
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.American journal of physiology. Lung cellular and molecular physiology · 2026Article
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.bioRxiv : the preprint server for biology · 2025Article
- Gain-of-function variants in SMAD4 compromise respiratory epithelial function.The Journal of allergy and clinical immunology · 2025Article
- Utilizing Neonatal Tracheal Aspirate-Derived Bronchial Basal Stem Cells to Model Respiratory Syncytial Virus Infection.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Activation of STAT3-mediated ciliated cell survival protects against severe infection by respiratory syncytial virus.The Journal of clinical investigation · 2024Article
- Airway Basal Stem Cells in COVID-19 Exhibit a Proinflammatory Signature and Impaired Mucocililary Differentiation.American journal of respiratory cell and molecular biology · 2024Article
- Single-Cell Profiling of Premature Neonate Airways Reveals a Continuum of Myeloid Differentiation.American journal of respiratory cell and molecular biology · 2023Article
- Airway Epithelial Cultures of Children with Esophageal Atresia as a Model to Study Respiratory Tract Disorders.Children (Basel, Switzerland) · 2023Article
- A Tracheal Aspirate-derived Airway Basal Cell Model Reveals a Proinflammatory Epithelial Defect in Congenital Diaphragmatic Hernia.American journal of respiratory and critical care medicine · 2023Article
- Perinatal origins of bronchopulmonary dysplasia-deciphering normal and impaired lung development cell by cell.Molecular and cellular pediatrics · 2023Review
- CircRNA, lncRNA, and mRNA profiles of umbilical cord blood exosomes from preterm newborns showing bronchopulmonary dysplasia.European journal of pediatrics · 2022Article
- Primary culture of tracheal aspirate-derived human airway basal stem cells.STAR protocols · 2022Article
- Stem Cell-derived Respiratory Epithelial Cell Cultures as Human Disease Models.American journal of respiratory cell and molecular biology · 2021Review
- Long-term differentiating primary human airway epithelial cell cultures: how far are we?Cell communication and signaling : CCS · 2021Review
- Prematurity alters the progenitor cell program of the upper respiratory tract of neonates.Scientific reports · 2021Article
- SIRT1-Related Signaling Pathways and Their Association With Bronchopulmonary Dysplasia.Frontiers in medicine · 2021Review
- Conditional cell reprogramming for modeling host-virus interactions and human viral diseases.Journal of medical virology · 2020Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBronchopulmonary dysplasia remains one of the most common complications of prematurity, despite significant improvements in perinatal care. Functional modeling of human lung development and disease, like BPD, is limited by our ability to access the lung and to maintain relevant progenitor cell populations in culture.
methodsWe supplemented Rho/SMAD signaling inhibition with mTOR inhibition to generate epithelial basal cell-like cell lines from tracheal aspirates of neonates.
resultsSingle-cell RNA-sequencing confirmed the presence of epithelial cells in tracheal aspirates obtained from intubated neonates. Using Rho/SMAD/mTOR triple signaling inhibition, neonatal tracheal aspirate-derived (nTAD) basal cell-like cells can be expanded long term and retain the ability to differentiate into pseudostratified airway epithelium.
conclusionsOur data demonstrate that neonatal tracheal aspirate-derived epithelial cells can provide a novel ex vivo human cellular model to study neonatal lung development and disease. IMPACT: Airway epithelial basal cell-like cell lines were derived from human neonatal tracheal aspirates. mTOR inhibition significantly extends in vitro proliferation of neonatal tracheal aspirate-derived basal cell-like cells (nTAD BCCs). nTAD BCCs can be differentiated into functional airway epithelium. nTAD BCCs provide a novel model to investigate perinatal lung development and diseases.
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