ArticlePLoS pathogens2020
The intergenic small non-coding RNA ittA is required for optimal infectivity and tissue tropism in Borrelia burgdorferi.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Identification of proteins exhibitingRNA biology · 2026Article
- TheInfection and immunity · 2026Article
- ThemBio · 2026Article
- DNA Binding by BosR Controls RpoS-Dependent and -Independent Gene Expression in Borrelia burgdorferi.Molecular microbiology · 2026Article
- Virulence Regulation inMicroorganisms · 2025Review
- Hitchhiker's Guide toJournal of bacteriology · 2024Review
- Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferi.Nature communications · 2023Article
- c-di-GMP regulates activity of the PlzA RNA chaperone from the Lyme disease spirochete.Molecular microbiology · 2023Article
- Borrelia burgdorferi Outer Surface Protein C Is Not the Sole Determinant of Dissemination in Mammals.Infection and immunity · 2023Article
- Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete.Nature communications · 2023Article
- Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogenbioRxiv : the preprint server for biology · 2023Article
- Deletion of a Genetic Region of lp17 Affects Plasmid Copy Number inFrontiers in cellular and infection microbiology · 2022Article
- Potential Regulatory Role in Mammalian Host Adaptation for a Small Intergenic Region of Lp17 in the Lyme Disease Spirochete.Frontiers in cellular and infection microbiology · 2022Article
- Live Imaging.Current issues in molecular biology · 2021Review
- Gene Regulation and Transcriptomics.Current issues in molecular biology · 2021Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Post-transcriptional regulation via small regulatory RNAs (sRNAs) has been implicated in diverse regulatory processes in bacteria, including virulence. One class of sRNAs, termed trans-acting sRNAs, can affect the stability and/or the translational efficiency of regulated transcripts. In this study, we utilized a collaborative approach that employed data from infection with the Borrelia burgdorferi Tn library, coupled with Tn-seq, together with borrelial sRNA and total RNA transcriptomes, to identify an intergenic trans-acting sRNA, which we designate here as ittA for infectivity-associated and tissue-tropic sRNA locus A. The genetic inactivation of ittA resulted in a significant attenuation in infectivity, with decreased spirochetal load in ear, heart, skin and joint tissues. In addition, the ittA mutant did not disseminate to peripheral skin sites or heart tissue, suggesting a role for ittA in regulating a tissue-tropic response. RNA-Seq analysis determined that 19 transcripts were differentially expressed in the ittA mutant relative to its genetic parent, including vraA, bba66, ospD and oms28 (bba74). Subsequent proteomic analyses also showed a significant decrease of OspD and Oms28 (BBA74) proteins. To our knowledge this is the first documented intergenic sRNA that alters the infectivity potential of B. burgdorferi.
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