ArticlePLoS pathogens2020
Structure and functional analysis of the Legionella pneumophila chitinase ChiA reveals a novel mechanism of metal-dependent mucin degradation.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- The immune-microbe-metal triad: a vicious cycle driving biomaterial corrosion in the oral inflammatory microenvironment.Bioactive materials · 2027Review
- GM000287 is a conserved determinant of serum resistance and virulence in Klebsiella pneumoniae.PLoS pathogens · 2026Article
- Major type IV pilin ofmBio · 2026Article
- Mucin stimulates the growth ofMicrobiology (Reading, England) · 2026Article
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Article
- Clostridium perfringens chitinases, key enzymes during early stages of necrotic enteritis in broiler chickens.PLoS pathogens · 2024Article
- The type II secretion system as an underappreciated and understudied mediator of interbacterial antagonism.Infection and immunity · 2024Review
- Exploring roles of the chitinase ChiC in modulatingMicrobiology spectrum · 2024Article
- The Legionella collagen-like protein employs a distinct binding mechanism for the recognition of host glycosaminoglycans.Nature communications · 2024Article
- Bacterial Chitinases and Their Role in Human Infection.Infection and immunity · 2023Review
- Salmonella-liberated dietary L-arabinose promotes expansion in superspreaders.Cell host & microbe · 2023Article
- Protein sociology of ProA, Mip and other secreted virulence factors at theFrontiers in cellular and infection microbiology · 2023Review
- Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis.BMC microbiology · 2022Article
- An Anaerobic Environment Drives the Harboring ofBiology · 2022Article
- Salmonella enterica serovar Typhimurium chitinases modulate the intestinal glycome and promote small intestinal invasion.PLoS pathogens · 2022Article
- Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation.NPJ biofilms and microbiomes · 2022Article
- Review
- Structural Characteristics and Immunomodulatory Effects of a Long-Chain Polysaccharide FromFrontiers in nutrition · 2022Article
- Pathogenicity and Virulence ofVirulence · 2021Review
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Chitinases are important enzymes that contribute to the generation of carbon and nitrogen from chitin, a long chain polymer of N-acetylglucosamine that is abundant in insects, fungi, invertebrates and fish. Although mammals do not produce chitin, chitinases have been identified in bacteria that are key virulence factors in severe respiratory, gastrointestinal and urinary diseases. However, it is unclear how these enzymes are able to carry out this dual function. Legionella pneumophila is the causative agent of Legionnaires' disease, an often-fatal pneumonia and its chitinase ChiA is essential for the survival of L. pneumophila in the lung. Here we report the first atomic resolution insight into the pathogenic mechanism of a bacterial chitinase. We derive an experimental model of intact ChiA and show how its N-terminal region targets ChiA to the bacterial surface after its secretion. We provide the first evidence that L. pneumophila can bind mucins on its surface, but this is not dependent on ChiA. This demonstrates that additional peripheral mucin binding proteins are also expressed in L. pneumophila. We also show that the ChiA C-terminal chitinase domain has novel Zn2+-dependent peptidase activity against mammalian mucin-like proteins, namely MUC5AC and the C1-esterase inhibitor, and that ChiA promotes bacterial penetration of mucin gels. Our findings suggest that ChiA can facilitate passage of L. pneumophila through the alveolar mucosa, can modulate the host complement system and that ChiA may be a promising target for vaccine development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.