ArticlePloS one2020
Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
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- Enzymatic, cellular breakdown and lysis in treatment of Beauveria Brongniartii on Spodoptera litura (Fabricius, 1775).Scientific reports · 2025Article
- Mosquito Gut Microbiota: A Review.Pathogens (Basel, Switzerland) · 2024Review
- Preliminary Bioactivity Assessment ofJournal of fungi (Basel, Switzerland) · 2024Article
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- Biocontrol efficacy of cajeput oil againstFrontiers in physiology · 2024Article
- Classification, biology and entomopathogenic fungi-based management and their mode of action againstFrontiers in microbiology · 2024Review
- Biology, classification, and entomopathogen-based management and their mode of action onFrontiers in microbiology · 2024Review
- Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence.Insects · 2023Review
- Essential oils fromHeliyon · 2023Article
- The Laboratory and Semi-Field Larvicidal Effects of Essential Oil Extracted fromJournal of parasitology research · 2023Article
- Entomopathogenic fungi based microbial insecticides and their physiological and biochemical effects onFrontiers in cellular and infection microbiology · 2023Article
- Toxicity of Metarhizium flavoviride conidia virulence against Spodoptera litura (Lepidoptera: Noctuidae) and its impact on physiological and biochemical activities.Scientific reports · 2022Article
- Metarhizium robertsii protease and conidia production, response to heat stress and virulence against Aedes aegypti larvae.AMB Express · 2021Article
- Insecticidal Efficacy of Microbial-Mediated Synthesized Copper Nano-Pesticide against Insect Pests and Non-Target Organisms.International journal of environmental research and public health · 2021Article
- Microbial Response to Fungal Infection in a Fungus-Growing Termite,Frontiers in microbiology · 2021Article
- Discovery of Novel Entomopathogenic Fungi for Mosquito-Borne Disease Control.Frontiers in fungal biology · 2021Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe fungal toxin acts as effective, low-cost chemical substances for pest control worldwide and also an alternative to synthetic insecticides. This study assessed the larvicidal potential of Metarhizium anisopliae fungi derived metabolites against Aedes aegypti, Anopheles stephensi, Culex quinquefasciatus and non-targeted organisms at 24hr post treatment.
methodIsolation of entomopathogenic fungi M. anisopliae from natural traps confirmed by using 18s rDNA biotechnological tools. Crude extracts from M. anisopliae solvent extraction and their secondary metabolites were bio-assayed following WHO standard procedures against Ae. aegypti, An. stephensi and Cx. quinquefasciatus, Artemia nauplii, Eudrilus eugeniae, and Solanum lycopersicum after 24 hr exposure. Histopathological analysis of E. eugeniae treated with fungi metabolites toxicity compared to those treated with Monocrotophos after 24hrpost-treatment. M. anisopliae metabolites were characterized using GC-MS and FT-IR analysis.
resultsThe larvicidal activity was recorded in highest concentration of 75μg/ml, with 85%, 97% and 89% mortality in Ae. aegypti, An. stephensi and Cx. quinquefasciatus respectively. M. anisopliae metabolites produced LC50 values in Ae. aegypti, 59.83μg/ml, in An. stephensi, 50.16μg/ml and in Cx. quinquefasciatus, 51.15μg/ml respectively. M. anisopliae metabolites produced lower toxic effects on A. nauplii, LC50 values were, 54.96μg/ml respectively. Bio-indicator toxicity results show 18% and 58% mortality was recorded in E. eugeniae and A. nauplii and also there is no phytotoxicity that was observed on S. lycopersicum L. under semi-field condition. E. eugeniae histopathological studies shows fungal metabolites showed lower sub-lethal effects compared to synthetic chemical pesticide at 24hrs of the treatment. The GC-MS and FT-IR analysis identified five major components of active ingredients.
conclusionFindings of this study indicate that, M. anisopliae ethyl acetate derived secondary metabolites are effective against larvae of Ae. aegypti, An. stephensi and Cx. quinquefasciatus mosquito species, lower toxicity effects were observed on non-target organisms such as, Artemia nauplii, Eudrilus eugeniae as well as, no toxicity effect were observed on Solanum lycopersicum. Further research should be conducted in laboratory for separation of single pure molecule and be tested semifield conditions.
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