Evidence map›Paper›PMID 32364315›Full record

ArticleJournal of fish diseases2020

Non-lethal loop-mediated isothermal amplification assay as a point-of-care diagnostics tool for Neoparamoeba perurans, the causative agent of amoebic gill disease.

Irene Cano, Robin McCullough, Brian Mulhearn, Susie Gunning, Ava Waine, Claire Joiner, Richard Paley

Abstract read
In one paragraph

Article in Journal of fish diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Irene CanoInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.ORCID https://orcid.org/0000-0001-8468-3707
Robin McCulloughInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.
Brian MulhearnInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.
Susie GunningInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.
Ava WaineInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.
Claire JoinerInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.
Richard PaleyInternational Centre of Excellence for Aquatic Animal Health, Cefas Weymouth Laboratory, Weymouth, UK.

Funding

Department for Environment, Food and Rural Affairs F7277H2020 European Research Council 634429
6 · The paper itself

Abstract

Neoparamoeba perurans is the causative agent of amoebic gill disease (AGD). Two loop-mediated isothermal amplification (LAMP) assays targeting the parasite 18S rRNA and the Atlantic salmon EF1α, used as internal control, were designed. The N. perurans LAMP assay did not amplify close relatives N. pemaquidensis and N. branchiphila, or the host DNA. This assay detected 10

Indexed as

Point-of-Care SystemsSalmo salarAmebiasisAmoebozoaAnimalsFish DiseasesGillsNucleic Acid Amplification Techniquesamoebic gill diseaseloop-mediated isothermal amplificationNeoparamoeba peruranspoint-of-care test

Identifiers

PMID32364315
PMCPMC7383609

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.