ArticleThe Plant journal : for cell and molecular biology2020
An isoleucine residue acts as a thermal and regulatory switch in wheat Rubisco activase.
Article in The Plant journal : for cell and molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 71 citations in OpenAlex.
- A Heat-Inducible Rubisco Activase Safeguards Photosynthesis via Multilevel Regulation and Dual Function.Plant biotechnology journal · 2026Article
- Overexpression of Rubisco Activase Improves Photosynthesis and Plant Growth in Rice Under Fluctuating Light Under Future High COPlant, cell & environment · 2026Article
- Two cowpea Rubisco activase isoforms for crop thermotolerance.The New phytologist · 2025Article
- A guide to understanding and measuring photosynthetic induction: considerations and recommendations.The New phytologist · 2025Review
- The heat is on: scaling improvements in photosynthetic thermal tolerance from the leaf to canopy to predict crop yields in a changing climate.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Needs and opportunities to future-proof crops and the use of crop systems to mitigate atmospheric change.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Systems analysis of long-term heat stress responses in the C4 grass Setaria viridis.The Plant cell · 2025Article
- Review
- SyntheticmicroPublication biology · 2025Article
- Review
- C4 grasses employ distinct strategies to acclimate rubisco activase to heat stress.Bioscience reports · 2024Article
- Review
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- Molecular characterization and expression pattern of Rubisco activase gene GhRCAβ2 in upland cotton (Gossypium hirsutum L.).Genes & genomics · 2024Article
- Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing.Plant direct · 2024Article
- Transgenic strategies to improve the thermotolerance of photosynthesis.Photosynthesis research · 2023Review
- Short- and long-term warming events on photosynthetic physiology, growth, and yields of field grown crops.The Biochemical journal · 2023Review
- Rubisco deactivation and chloroplast electron transport rates co-limit photosynthesis above optimal leaf temperature in terrestrial plants.Nature communications · 2023Article
- Effects ofPlants (Basel, Switzerland) · 2023Article
- Temperature-induced changes in Arabidopsis Rubisco activity and isoform expression.Journal of experimental botany · 2023Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of Rubisco, the gatekeeper of carbon fixation into the biosphere, by its molecular chaperone Rubisco activase (Rca) is essential for photosynthesis and plant growth. Using energy from ATP hydrolysis, Rca promotes the release of inhibitors and restores catalytic competence to Rubisco-active sites. Rca is sensitive to moderate heat stress, however, and becomes progressively inhibited as the temperature increases above the optimum for photosynthesis. Here, we identify a single amino acid substitution (M159I) that fundamentally alters the thermal and regulatory properties of Rca in bread wheat (Triticum aestivum L.). Using site-directed mutagenesis, we demonstrate that the M159I substitution extends the temperature optimum of the most abundant Rca isoform by 5°C in vitro, while maintaining the efficiency of Rubisco activation by Rca. The results suggest that this single amino acid substitution acts as a thermal and regulatory switch in wheat Rca that can be exploited to improve the climate resilience and efficiency of carbon assimilation of this cereal crop as temperatures become warmer and more volatile.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.