ArticleJournal of assisted reproduction and genetics2020
"Universal Warming" protocol for vitrified oocytes to streamline cell exchange for transnational donation programs: a multi-center study.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Multi-omics and developmental comparison of direct and conventional warming methods in vitrified human and mouse cleavage-stage embryos.Human reproduction (Oxford, England) · 2026Article
- Beyond aneuploidy: embryo morphology, maternal age, and miscarriage dynamics in PGT-A selected single embryo transfer.Journal of assisted reproduction and genetics · 2026Article
- Equivalent outcomes of human oocytes after vitrification or slow freezing with a modified rehydration protocol.Reproductive biology and endocrinology : RB&E · 2025Article
- Application of a single "Universal warming protocol" for vitrified donor oocytes: A multicenter study.Journal of assisted reproduction and genetics · 2025Article
- Live birth outcomes following single-step blastocyst warming technique - optimizing efficiency without impacting live birth rates.Journal of assisted reproduction and genetics · 2024Article
- Comparative Assessment of Survival and Clinical Outcome Between Two Commercial Vitrification Kits with Different Warming Protocols After Blastocyst Culture: Potential Perspectives Toward Simplified Warming Procedures.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Optimizing the Ovarian Tissue Cryopreservation in the 'Oncofertility' Institutional Program at an Italian National Cancer Institute.Healthcare (Basel, Switzerland) · 2023Article
- The international transportation of frozen embryos does not affect IVF outcomes.Archives of gynecology and obstetrics · 2023Article
- An all-37 °C thawing method improves the clinical outcomes of vitrified frozen-thawed embryo transfer: a retrospective study using a case-control matching analysis.Archives of gynecology and obstetrics · 2023Article
- The effect of high-throughput vitrification of human ovarian cortex tissue on follicular viability: a promising alternative to conventional slow freezing?Archives of gynecology and obstetrics · 2023Article
- Are commercial warming kits interchangeable for vitrified human blastocysts? Further evidence for the adoption of a Universal Warming protocol.Journal of assisted reproduction and genetics · 2022Article
- "Universal Warming" protocol for vitrified oocytes to streamline cell exchange for transnational donation programs: a multi-center study.Journal of assisted reproduction and genetics · 2020Article
- A monocentric analysis of the efficacy of extracellular cryoprotectants in unfrozen solutions for cleavage stage embryos.Reproductive biology and endocrinology : RB&E · 2019Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the clinical efficacy of a "Universal Warming" protocol, based on subsequent steps with 1 M and 0.5 M concentration of extracellular cryoprotectant (ECCP), on shipped oocytes. Oocytes are vitrified using different brands of ready-to-use kits which recommend that the use of their own warming kit and combining different vitrification/warming kits may have legal consequences for assisted reproductive (AR) centers, until this practice has been validated with clinical studies.
methodsRetrospective multi-center transnational observational study. Number of oocytes warmed 1.898. Vitrification performed with vitrification kit (Kitazato, Japan); warming carried out randomly with two different kits: Kitazato warming kit and Vit Kit®-Thaw (FujiFilm Irvine, USA). Warmed oocytes were assigned to 2 groups: KK (Kitazato/Kitazato) 939, and KI (Kitazato/Irvine) 959. Primary endpoint: survival rate. Secondary endpoints: fertilization rate; blastulation rate; implantation rate; live birth rate.
resultsSurvival was comparable between the groups: 84.6% (795/939) in group KK vs 82.1% (787/959) in group KI. Fertilization rate was lower (P = 0.027) in group KK (75.7%-602/795) than in group KI (80.4%-633/787). Blastulation and implantation and live birth rates were all statistically comparable between the study groups: blastulation rate was 58.5% (352/602) vs 57.8% (366/633); implantation rate was 41.5% (80/193) vs 45.9% (84/183); live birth rate was 52.5% (62/118) in KK and 45.0% (54/120) in KI.
conclusionThe use of this "Universal Warming" protocol simplifies vitrified oocyte exchange between AR centers in different countries, and overcomes potential regulatory/commercial/availability differences affecting clinical practice.
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