Evidence map›Paper›PMID 32362951›Full record

ArticleChemCatChem2020

An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation.

Aşkın S Aslan-Üzel, Andy Beier, David Kovář, Clemens Cziegler, Santosh K Padhi, Eva D Schuiten, Mark Dörr, Dominique Böttcher, Frank Hollmann, Florian Rudroff and 6 more

Open access · hybridAbstract read
In one paragraph

Article in ChemCatChem, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Enzymatic Photometric Assays for the Selective Detection of Halides.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  6. From Natural Methylation to Versatile Alkylations Using Halide Methyltransferases.Chembiochem : a European journal of chemical biology · 2021
    Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 5 countries.

Aşkın S Aslan-ÜzelDepartment of Biotechnology & Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald 17487 Germany.ORCID 0000-0002-1157-9391
Andy BeierLoschmidt Laboratories Department of Experimental Biology and RECETOX Faculty of Science Masaryk University Brno 625 00 Czech Republic.
David KovářLoschmidt Laboratories Department of Experimental Biology and RECETOX Faculty of Science Masaryk University Brno 625 00 Czech Republic.
Clemens CzieglerInstitute of Applied Synthetic Chemistry TU Wien Vienna 1060 Austria.
Santosh K PadhiBiocatalysis and Enzyme Engineering Laboratory Department of Biochemistry School of Life Sciences University of Hyderabad Gachibowli 500046 India.
Eva D SchuitenDepartment of Biotechnology & Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald 17487 Germany.
Mark DörrDepartment of Biotechnology & Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald 17487 Germany.
Dominique BöttcherDepartment of Biotechnology & Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald 17487 Germany.
Frank HollmannDepartment of Biotechnology Delft University of Technology Delft 2629 HZ (The Netherlands.
Florian RudroffInstitute of Applied Synthetic Chemistry TU Wien Vienna 1060 Austria.
Marko D MihovilovicInstitute of Applied Synthetic Chemistry TU Wien Vienna 1060 Austria.
Tomáš BuryškaLoschmidt Laboratories Department of Experimental Biology and RECETOX Faculty of Science Masaryk University Brno 625 00 Czech Republic.
Jiří DamborskýLoschmidt Laboratories Department of Experimental Biology and RECETOX Faculty of Science Masaryk University Brno 625 00 Czech Republic.ORCID 0000-0002-7848-8216
Zbyněk ProkopLoschmidt Laboratories Department of Experimental Biology and RECETOX Faculty of Science Masaryk University Brno 625 00 Czech Republic.ORCID 0000-0001-9358-4081
Christoffel P S BadenhorstDepartment of Biotechnology & Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald 17487 Germany.ORCID 0000-0002-5874-4577
Uwe T BornscheuerDepartment of Biotechnology & Enzyme Catalysis Institute of Biochemistry Greifswald University Greifswald 17487 Germany.ORCID 0000-0003-0685-2696
Universitätsmedizin Greifswald · DEMasaryk University · CZTU Wien · ATDelft University of Technology · NLUniversity of Hyderabad · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Halide assays are important for the study of enzymatic dehalogenation, a topic of great industrial and scientific importance. Here we describe the development of a very sensitive halide assay that can detect less than a picomole of bromide ions, making it very useful for quantifying enzymatic dehalogenation products. Halides are oxidised under mild conditions using the vanadium-dependent chloroperoxidase from

Indexed as

dehalogenasefluorescencehalideshaloalkanehaloperoxidase

Identifiers

PMID32362951
PMCPMC7188320
OpenAlexW2995783271

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.