ArticleJournal of assisted reproduction and genetics2020
Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering.
Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- The matrisome, methods, analytical evaluation and potential of decellularized porcine ovaries for fertility preservation.Cell and tissue research · 2026Review
- Engineering ovarian tissue via biofabrication and 3D bioprinting: Challenges and emerging perspectives.Bioengineering & translational medicine · 2026Review
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Review
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- Generation of Tailored Extracellular Matrix Hydrogels for the Study of In Vitro Folliculogenesis in Response to Matrisome-Dependent Biochemical Cues.Bioengineering (Basel, Switzerland) · 2024Article
- Review
- Generation of bovine decellularized testicular bio-scaffolds as a 3D platform for testis bioengineering.Frontiers in bioengineering and biotechnology · 2024Article
- 3D culture applied to reproduction in females: possibilities and perspectives.Animal reproduction · 2024Review
- Article
- 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells.International journal of molecular sciences · 2023Article
- Advanced bioengineering of female germ cells to preserve fertility.Biology of reproduction · 2022Review
- Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice.Journal of functional biomaterials · 2022Article
- Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models.Journal of assisted reproduction and genetics · 2022Article
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine.International journal of molecular sciences · 2022Review
- Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds.Nanomaterials (Basel, Switzerland) · 2022Article
- Characteristics of a decellularized human ovarian tissue created by combined protocols and its interaction with human endometrial mesenchymal cells.Progress in biomaterials · 2021Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
purposeTo develop a new protocol for whole-ovary decellularization for the production of a 3D bioscaffold suitable for in vitro/ex vivo studies and for the reconstruction of a bioengineered ovary.
methodsPorcine ovaries were subjected to the decellularization process (DECELL; n = 20) that involved a freeze-thaw cycle, followed by sequential incubations in 0.5% SDS for 3 h, 1% Triton X-100 for 9 h, and 2% deoxycholate for 12 h. Untreated ovaries were used as a control (CTR; n = 6). Both groups were analyzed to evaluate cell and DNA removal as well as ECM preservation. DECELL bioscaffolds were assessed for cytotoxicity and cell homing ability.
resultsDECELL ovaries maintained shape and homogeneity without any deformation, while their color turned from red to white. Histological staining and DNA quantification confirmed a decrease of 98.11% in DNA content, compared with the native tissue (CTR). Histochemical assessments demonstrated the preservation of intact ECM microarchitecture after the decellularization process. This was also confirmed by quantitative analysis of collagen, elastin, and GAG contents. DECELL bioscaffold showed no cytotoxic effects in co-culture and, when re-seeded with homologous fibroblasts, encouraged a rapid cell adhesion and migration, with repopulating cells increasing in number and aggregating in cluster-like structures, consistent with its ability to sustain cell adherence, proliferation, and differentiation.
conclusionThe protocol described allows for the generation of a 3D bioscaffold that may constitute a suitable model for ex vivo culture of ovarian cells and follicles, as well as a promising tool for the reconstruction of a bioengineered ovary.
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Registered trials
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