Evidence map›Paper›PMID 32361827›Full record

ArticleTranslational stroke research2021

Extracellular Vesicles Derived from Neural Progenitor Cells--a Preclinical Evaluation for Stroke Treatment in Mice.

X Zheng, L Zhang, Y Kuang, V Venkataramani, F Jin, K Hein, M P Zafeiriou, C Lenz, W Moebius, E Kilic and 6 more

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational stroke research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 63 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed, 4 pooled it
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 4 syntheses or guidelines pooled it, 82 citations in OpenAlex.

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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 7 institutions in 3 countries.

X ZhengDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany.
L ZhangDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany.
Y KuangDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany.
V VenkataramaniInstitute of Pathology, University Medical Center Goettingen, Goettingen, Germany.
F JinDepartment of Hematology, Cancer Center, The First Hospital of Jilin University, Changchun, Jilin, China.
K HeinDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany.
M P ZafeiriouInstitute for Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany.
C LenzBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany.
W MoebiusDepartment of Neurogenetics, Electron Microscopy Group, Max Planck Institute of Experimental Medicine, Goettingen, Germany.
E KilicRegenerative and Restorative Medical Research Center, Istanbul Medipol University, Istanbul, Turkey.
D M HermannDepartment of Neurology, University of Duisburg-Essen Medical School, Essen, Germany.
M S WeberDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany.
H UrlaubBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany.
W-H ZimmermannInstitute for Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany.
M BährDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany.
Thorsten R DoeppnerDepartment of Neurology, University Medical Center Goettingen, Robert-Koch-Str. 40, 37075, Goettingen, Germany. thorsten.doeppner@med.uni-goettingen.de.
University of Göttingen · DEGerman Centre for Cardiovascular Research · DEIstanbul Medipol University · TRMax Planck Institute for Biophysical Chemistry · DEJilin University · CNMax Planck Institute of Experimental Medicine · DEUniversity of Duisburg-Essen · DE

Funding

Deutsche Forschungsgemeinschaft (DE) EXC 2067/1- 390729940Deutsche Forschungsgemeinschaft (DE) ZI 708/10-1; CRC 1002 C04 and S01; IRTG1816 RP12; EXC 2067/1- 390729940Fondation Leducq 14CVD04
6 · The paper itself

Abstract

Stem cells such as mesenchymal stem cells (MSCs) enhance neurological recovery in preclinical stroke models by secreting extracellular vesicles (EVs). Since previous reports have focused on the application of MSC-EVs only, the role of the most suitable host cell for EV enrichment and preclinical stroke treatment remains elusive. The present study aimed to evaluate the therapeutic potential of EVs derived from neural progenitor cells (NPCs) following experimental stroke. Using the PEG technique, EVs were enriched and characterized by electron microscopy, proteomics, rt-PCR, nanosight tracking analysis, and Western blotting. Different dosages of NPC-EVs displaying a characteristic profile in size, shape, cargo protein, and non-coding RNA contents were incubated in the presence of cerebral organoids exposed to oxygen-glucose deprivation (OGD), significantly reducing cell injury when compared with control organoids. Systemic administration of NPC-EVs in male C57BL6 mice following experimental ischemia enhanced neurological recovery and neuroregeneration for as long as 3 months. Interestingly, the therapeutic impact of such NPC-EVs was found to be not inferior to MSC-EVs. Flow cytometric analyses of blood and brain samples 7 days post-stroke demonstrated increased blood concentrations of B and T lymphocytes after NPC-EV delivery, without affecting cerebral cell counts. Likewise, a biodistribution analysis after systemic delivery of NPC-EVs revealed the majority of NPC-EVs to be found in extracranial organs such as the liver and the lung. This proof-of-concept study supports the idea of EVs being a general concept of stem cell-induced neuroprotection under stroke conditions, where EVs contribute to reverting the peripheral post-stroke immunosuppression.

Indexed as

Disease Models, AnimalAnimalsAnimals, NewbornCells, CulturedExtracellular VesiclesMaleMiceMice, Inbred C57BLNeural Stem CellsOrganoidsStrokeTreatment OutcomeCerebral ischemiaExtracellular vesicles, neural progenitor cellsNeurological recoveryNeuroregeneration

Identifiers

PMID32361827
PMCPMC7803677
OpenAlexW3022817183

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.