ArticleMethods in enzymology2020
Analysis of vitamin A and retinoids in biological matrices.
Article in Methods in enzymology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Standardising Culture Medium Safety Testing for Cultivated Meat: Outputs from a Workshop and Case Study.Foods (Basel, Switzerland) · 2026Article
- Targeting autocrine retinoic acid signaling by ALDH1A2 inhibition enhances antitumor dendritic cell vaccine efficacy.Nature immunology · 2026Article
- The Influences of RARγ on the Behavior of Normal and Cancer Stem Cells.International journal of molecular sciences · 2026Review
- Differential retinoic acid responses across testicular developmentbioRxiv : the preprint server for biology · 2025Article
- Graphene-polymer nanofibers enable optically induced electrical responses in stem cell-derived electrically excitable cells and brain organoids.Biomaterials · 2025Article
- Disease modifying biomaterials for modulating mechanical allodynia in a preclinical model of rheumatoid arthritis.Bioengineering & translational medicine · 2025Article
- All-trans-retinal as a modulator of metabolic and mitochondrial signatures in non-tumorigenic and malignant breast cells: a Raman-based approach.Mikrochimica acta · 2025Article
- Regional heterogeneity of the blood-brain barrier.Nature communications · 2025Article
- Sex and Age but Not Body Mass Index (BMI) Predict Serum Retinol Binding Protein 4 (RBP4) and Transthyretin (TTR) Concentrations.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Inhibition of heme-thiolate monooxygenase CYP1B1 prevents hepatic stellate cell activation and liver fibrosis by accumulating trehalose.Science translational medicine · 2024Article
- Article
- LX-2 Stellate Cells Are a Model System for Investigating the Regulation of Hepatic Vitamin A Metabolism and Respond to Tumor Necrosis FactorDrug metabolism and disposition: the biological fate of chemicals · 2024Article
- Effect of isotretinoin on CYP2D6 and CYP3A activity in patients with severe acne.British journal of clinical pharmacology · 2024Article
- How Can Selected Dietary Ingredients Influence the Development and Progression of Endometriosis?Nutrients · 2024Review
- Raising NADCirculation · 2023Article
- Temporal changes in the systemic concentrations of retinoids in pregnant and postpartum women.PloS one · 2023Article
- Retinoic acid receptor regulation of decision-making for cell differentiation.Frontiers in cell and developmental biology · 2023Review
- Gut commensals expand vitamin A metabolic capacity of the mammalian host.Cell host & microbe · 2022Article
- Plasma Retinoid Concentrations Are Altered in Pregnant Women.Nutrients · 2022Article
- Altered vitamin A metabolism in human liver slices corresponds to fibrogenesis.Clinical and translational science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Vitamin A signaling pathways are predominantly driven by the cellular concentrations of all-trans-retinoic acid (atRA), as the main mechanism of retinoid signaling is via activation of retinoic acid receptors. atRA concentrations are in turn controlled by the storage of vitamin A and enzymatic processes that synthesize and clear atRA. This has resulted in the need for robust and highly specific analytical methods to accurately quantify retinoids in diverse biological matrices. Tissue-specific differences in both the quantity of retinoids and background matrix interferences can confound the quantification of retinoids, and the bioanalysis requires high performance instrumentation, such as liquid chromatography mass-spectrometry (LC-MS). Successful bioanalysis of retinoids is further complicated by the innate structural instability of retinoids and their relatively high lipophilicity. Further, in vitro experiments with retinoids require attention to experimental design and interpretation to account for the instability of retinoids due to isomerization and degradation, sequential metabolism to numerous structurally similar metabolites, and substrate depletion during experiments. In addition, in vitro biological activity is often confounded by residual presence of retinoids in common biological reagents such as cell culture media. This chapter identifies common biological and analytical complexities in retinoid bioanalysis in diverse biological matrices, and in the use of retinoids in cell culture and metabolic incubations. In addition, this chapter highlights best practices for the successful detection and quantification of the vitamin A metabolome in a wide range of biological matrices.
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Registered trials
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