Evidence map›Paper›PMID 32359364›Full record

ArticleJournal of ovarian research2020

The differential expression of miRNAs between ovarian endometrioma and endometriosis-associated ovarian cancer.

Natsuho Nakamura, Yoshito Terai, Misa Nunode, Kana Kokunai, Hiromi Konishi, Sayaka Taga, Mayumi Nakamura, Masae Yoo, Masami Hayashi, Yoshiki Yamashita and 1 more

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In one paragraph

Article in Journal of ovarian research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Natsuho NakamuraDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan. gyn137@osaka-med.ac.jp.
Yoshito TeraiDepartment of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan.
Misa NunodeDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
Kana KokunaiDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
Hiromi KonishiDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
Sayaka TagaDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
Mayumi NakamuraHirakata City Hospital, Osaka, Japan.
Masae YooDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
Masami HayashiDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.
Yoshiki YamashitaUmeda Fertility Clinic, Osaka, Japan.
Masahide OhmichiDepartment of obstetrics and gynecology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki, Osaka, 569-8686, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroRNAs (miRNAs) have been implicated to play a vital role in development, differentiation, cell proliferation and apoptosis. However, which miRNAs are actually associated with endometriosis-associated ovarian cancer remains controversial.

methodsSerum and ascites samples were obtained from all patients. Serum samples from 5 cases of ovarian endometrioma and endometriosis-associated ovarian cancer each were submitted for comprehensive miRNA microarray profiling. We investigated the differential expression of miRNAs between the two groups to confirm the pivotal role of miRNAs. Quantitative reverse transcription-polymerase chain reaction validation of five selected miRNAs [miR-92a-3p, miR-486-5p, miR-4484, miR-6821-5p, and miR-7108-5p] was performed, and miR-486-5p expression analysis was followed by proliferation and wound healing assays, depending on the expression of miR-486-5p.

resultmiR-486-5p expression in serum and ascites samples from endometriosis-associated ovarian cancer patients was significantly higher than that from ovarian endometrioma patients. Moreover, the miR-486-5p level in serum and ascites samples was significantly correlated with the severity of the endometriosis. The upregulation of miR-486-5p in immortalized ovarian endometrioma cells significantly increased proliferation and migration. In contrast, the downregulation of miR-486-5p in these cells significantly decreased proliferation and migration.

conclusionmiR-486-5p might function as an oncogenic miRNA in endometriosis-associated ovarian cancer and could be a noninvasive biomarker to prospect the severity of ovarian endometrioma.

Indexed as

MicroRNAsAdultAscitesBiomarkers, TumorCell LineCell ProliferationEndometriosisFemaleHumansMiddle AgedOvarian NeoplasmsWound HealingBiomarkers, TumorMicroRNAsMIRN486 microRNA, humanEndometriosis-associated ovarian cancermiR-486-5pmiRNA microarrayOvarian endometrioma

Identifiers

PMID32359364
PMCPMC7196233

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.