ArticlePlant biotechnology journal2020
Loss of function of CRT1a (calreticulin) reduces plant susceptibility to Verticillium longisporum in both Arabidopsis thaliana and oilseed rape (Brassica napus).
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 80 citations in OpenAlex.
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- Next-Generation Precision Breeding in Peanut (Insects · 2026Review
- Transcriptomic Analysis Reveals Candidate Hub Genes and Putative Pathways inCurrent issues in molecular biology · 2025Article
- Review
- Unlocking genetic potential: a review of the role of CRISPR/Cas technologies in rapeseed improvement.Stress biology · 2025Review
- All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Identification of the CaCRT gene family and function of CaCRT1 under low-temperature stress in pepper (Capsicum annuum L.).Scientific reports · 2025Article
- Advances in genomic tools for plant breeding: harnessing DNA molecular markers, genomic selection, and genome editing.Biological research · 2024Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Insights into the early transcriptomic response against watermelon mosaic virus in melon.BMC plant biology · 2024Article
- Genomic insights intoFrontiers in microbiology · 2024Review
- Comprehensive genomic characterization and expression analysis of calreticulin gene family in tomato.Frontiers in plant science · 2024Article
- Modification of Fatty Acid Profile and Oil Contents Using Gene Editing in Oilseed Crops for a Changing Climate.GM crops & food · 2023Review
- Reproducibility of next-generation-sequencing-based analysis of a CRISPR/Cas9 genome edited oil seed rape.Food chemistry. Molecular sciences · 2023Article
- Specificity Testing for NGT PCR-Based Detection Methods in the Context of the EU GMO Regulations.Foods (Basel, Switzerland) · 2023Article
- Targeted genome editing in polyploids: lessons fromFrontiers in plant science · 2023Review
- A highly efficient genetic transformation system for broccoli and subcellular localization.Frontiers in plant science · 2023Article
- Engineering plant immune circuit: walking to the bright future with a novel toolbox.Plant biotechnology journal · 2023Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassica napus is highly susceptible towards Verticillium longisporum (Vl43) with no effective genetic resistance. It is believed that the fungus reprogrammes plant physiological processes by up-regulation of so-called susceptibility factors to establish a compatible interaction. By transcriptome analysis, we identified genes, which were activated/up-regulated in rapeseed after Vl43 infection. To test whether one of these genes is functionally involved in the infection process and loss of function would lead to decreased susceptibility, we firstly challenged KO lines of corresponding Arabidopsis orthologs with Vl43 and compared them with wild-type plants. Here, we report that the KO of AtCRT1a results in drastically reduced susceptibility of plants to Vl43. To prove crt1a mutation also decreases susceptibility in B. napus, we identified 10 mutations in a TILLING population. Three T3 mutants displayed increased resistance as compared to the wild type. To validate the results, we generated CRISPR/Cas-induced BnCRT1a mutants, challenged T2 plants with Vl43 and observed an overall reduced susceptibility in 3 out of 4 independent lines. Genotyping by allele-specific sequencing suggests a major effect of mutations in the CRT1a A-genome copy, while the C-genome copy appears to have no significant impact on plant susceptibility when challenged with Vl43. As revealed by transcript analysis, the loss of function of CRT1a results in activation of the ethylene signalling pathway, which may contribute to reduced susceptibility. Furthermore, this study demonstrates a novel strategy with great potential to improve plant disease resistance.
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