ArticleProceedings of the National Academy of Sciences of the United States of America2020
Genome organization and interaction with capsid protein in a multipartite RNA virus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- A PD-L1 Nanobody-Engineered Synthetic RNA Virus Enhances Hepatocellular Carcinoma Immunotherapy by Interfering PCSK9 and Upregulating MHC-I.Exploration (Beijing, China) · 2026Article
- Packaging of Single-Stranded RNA in Viruses and Virus-Like Particles.Annual review of biochemistry · 2026Review
- Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.European biophysics journal : EBJ · 2026Review
- Rapid Solar Photoinactivation of Influenza Virus and Phi6 in Colored Surface Water.Environmental science & technology · 2026Article
- Asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus.Science advances · 2025Article
- Computational biophysical characterization of a superradiant virus-like particle in its ground state.bioRxiv : the preprint server for biology · 2025Article
- Disassembly of Virus-Like Particles and the Stabilizing Role of the Nucleic Acid Cargo.The journal of physical chemistry. B · 2025Article
- Macromolecular Cargo Encapsulation via In Vitro Assembly of Two-Component Protein Nanoparticles.Advanced healthcare materials · 2024Article
- The Basic Architecture of Viruses.Sub-cellular biochemistry · 2024Review
- Modulation of Capsid Dynamics in Bromoviruses by the Host and Heterologous Viral Replicase.Journal of virology · 2023Article
- Assembly of Protein Cages for Drug Delivery.Pharmaceutics · 2022Review
- Single-particle studies of the effects of RNA-protein interactions on the self-assembly of RNA virus particles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Packaging contests between viral RNA molecules and kinetic selectivity.PLoS computational biology · 2022Article
- The diversity of protein-protein interaction interfaces within T=3 icosahedral viral capsids.Frontiers in molecular biosciences · 2022Article
- Virus-like nanoparticles as a theranostic platform for cancer.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Subset of Fluorophores Is Responsible for Radiation Brightening in Viromimetic Particles.The journal of physical chemistry. B · 2021Article
- Relationships between RNA topology and nucleocapsid structure in a model icosahedral virus.Biophysical journal · 2021Article
- Review
- Structural 3D Domain Reconstruction of the RNA Genome from Viruses with Secondary Structure Models.Viruses · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
We report the asymmetric reconstruction of the single-stranded RNA (ssRNA) content in one of the three otherwise identical virions of a multipartite RNA virus, brome mosaic virus (BMV). We exploit a sample consisting exclusively of particles with the same RNA content-specifically, RNAs 3 and 4-assembled in planta by agrobacterium-mediated transient expression. We find that the interior of the particle is nearly empty, with most of the RNA genome situated at the capsid shell. However, this density is disordered in the sense that the RNA is not associated with any particular structure but rather, with an ensemble of secondary/tertiary structures that interact with the capsid protein. Our results illustrate a fundamental difference between the ssRNA organization in the multipartite BMV viral capsid and the monopartite bacteriophages MS2 and Qβ for which a dominant RNA conformation is found inside the assembled viral capsids, with RNA density conserved even at the center of the particle. This can be understood in the context of the differing demands on their respective lifecycles: BMV must package separately each of several different RNA molecules and has been shown to replicate and package them in isolated, membrane-bound, cytoplasmic complexes, whereas the bacteriophages exploit sequence-specific "packaging signals" throughout the viral RNA to package their monopartite genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.