ArticleProceedings of the National Academy of Sciences of the United States of America2020
Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 122 citations in OpenAlex.
- A systematic review of ultrasound-mediated drug delivery to the eye and critical insights to facilitate a timely path to the clinic.Theranostics · 2023Pooled it
- LED-Modulated Plasmoporation for Highly Efficient Intracellular Cargo Delivery to Suspension Cells.Nano letters · 2026Article
- Parallelized droplet microfluidic mechanoporation enables robust and clogging-resistant intracellular gene delivery.Microsystems & nanoengineering · 2026Article
- Reconfigurable Acoustofluidic Microvortices for Selective Microcargo Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Streamlined Fabrication and Acoustofluidic Purification of Silver-Decorated Polystyrene Microspheres (PS-AgNPs) for SERS Applications.ACS applied nano materials · 2026Article
- Precision acoustofluidics for high-throughput mechanobiology in suspension cells.Science advances · 2026Article
- Nanotechnology for CAR T cells and tumour-infiltrating lymphocyte therapies.Nature nanotechnology · 2025Review
- The Rise of Mechanobiology for Advanced Cell Engineering and Manufacturing.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Microfluidic Platforms for Ex Vivo and In Vivo Gene Therapy.Biosensors · 2025Review
- Acoustic technologies for the orchestration of cellular functions for therapeutic applications.Science advances · 2025Review
- Revolutionising Cancer Immunotherapy: Advancements and Prospects in Non-Viral CAR-NK Cell Engineering.Cell proliferation · 2025Review
- Acoustofluidics-Based Intracellular Nanoparticle Delivery.Engineering (Beijing, China) · 2025Article
- Influence of cell shape on sonoporation efficiency in microbubble-facilitated delivery using micropatterned cell arrays.Scientific reports · 2024Article
- Acoustofluidic tweezers via ring resonance.Science advances · 2024Article
- Acoustothermal transfection for cell therapy.Science advances · 2024Article
- Acoustofluidic Actuation of Living Cells.Micromachines · 2024Review
- High throughput intracellular delivery by viscoelastic mechanoporation.Nature communications · 2024Article
- Cavity-agnostic acoustofluidic manipulations enabled by guided flexural waves on a membrane acoustic waveguide actuator.Microsystems & nanoengineering · 2024Article
- Fluorinated Silane-Modified Filtroporation Devices Enable Gene Knockout in Human Hematopoietic Stem and Progenitor Cells.ACS applied materials & interfaces · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
19 authors at 4 institutions in 1 country.
Funding
Abstract
Advances in gene editing are leading to new medical interventions where patients' own cells are used for stem cell therapies and immunotherapies. One of the key limitations to translating these treatments to the clinic is the need for scalable technologies for engineering cells efficiently and safely. Toward this goal, microfluidic strategies to induce membrane pores and permeability have emerged as promising techniques to deliver biomolecular cargo into cells. As these technologies continue to mature, there is a need to achieve efficient, safe, nontoxic, fast, and economical processing of clinically relevant cell types. We demonstrate an acoustofluidic sonoporation method to deliver plasmids to immortalized and primary human cell types, based on pore formation and permeabilization of cell membranes with acoustic waves. This acoustofluidic-mediated approach achieves fast and efficient intracellular delivery of an enhanced green fluorescent protein-expressing plasmid to cells at a scalable throughput of 200,000 cells/min in a single channel. Analyses of intracellular delivery and nuclear membrane rupture revealed mechanisms underlying acoustofluidic delivery and successful gene expression. Our studies show that acoustofluidic technologies are promising platforms for gene delivery and a useful tool for investigating membrane repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.