ArticleJournal of the American Society of Nephrology : JASN2020
Quantitative Proteomics of All 14 Renal Tubule Segments in Rat.
Article in Journal of the American Society of Nephrology : JASN, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 131 citations in OpenAlex.
- Trial
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- GLP-1 receptor agonists and next-generation metabolic hormone therapies in chronic kidney disease.Nature reviews. Nephrology · 2026Review
- Cellular microenvironment of erythropoietin-producing cells in hypoxic and injured mouse kidneys.Experimental physiology · 2026Article
- Analysis of Plasma Extracellular Vesicles in Normal-Weight and Overweight Type 2 Diabetes Mellitus Using Multimodal SERS and RNA-Seq.bioRxiv : the preprint server for biology · 2026Article
- Biguanides are associated with decreased early mortality and risk of acute kidney injury in hospitalised patients with COVID-19: a nationwide retrospective cohort study in Japan.Clinical and experimental nephrology · 2026Article
- Na/K-ATPase in the renal proximal tubule cell: classic & novel roles in sodium handling.Frontiers in physiology · 2026Review
- The lipidome of the kidney tubules of ob/ob mice is affected at the infralesional level.Cell death discovery · 2025Article
- KCC3 is not required for the distal convoluted tubule response to reduced dietary potassium intake.American journal of physiology. Renal physiology · 2025Article
- Decellularized rat tubules for tissue engineering.Translational andrology and urology · 2025Article
- Utilizing Omic Data to Understand Integrative Physiology.Physiology (Bethesda, Md.) · 2025Review
- NRBP1 and TSC22D proteins affect distal convoluted tubule physiology through modulation of the WNK pathway.Science advances · 2025Article
- Roadblocks of Urinary EV Biomarkers: Moving Toward the Clinic.Journal of extracellular vesicles · 2025Review
- Glycolytic control proteins in urinary extracellular vesicles are elevated during kidney transplant T cell-mediated rejection.BMC nephrology · 2025Observational
- Human and rat renal proximal tubule in vitro models for ADME applications.Archives of toxicology · 2025Review
- Analysis of human urinary extracellular vesicles reveals disordered renal metabolism in myotonic dystrophy type 1.Nature communications · 2025Article
- A brief history of the cortical thick ascending limb: a systems-biology perspective.American journal of physiology. Renal physiology · 2025Review
- Western diet exacerbates a murine model of Balkan nephropathy.American journal of physiology. Renal physiology · 2025Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPrevious research has used RNA sequencing in microdissected kidney tubules or single cells isolated from the kidney to profile gene expression in each type of kidney tubule epithelial cell. However, because proteins, not mRNA molecules, mediate most cellular functions, it is desirable to know the identity and amounts of each protein species to understand function. Recent improvements in the sensitivity of mass spectrometers offered us the ability to quantify the proteins expressed in each of 14 different renal tubule segments from rat.
methodsWe manually dissected kidney tubules from rat kidneys and subjected samples to protein mass spectrometry. We used the "proteomic ruler" technique to estimate the number of molecules of each protein per cell.
resultsOver the 44 samples analyzed, the average number of quantified proteins per segment was 4234, accounting for at least 99% of protein molecules in each cell. We have made the data publicly available online at the Kidney Tubule Expression Atlas website (https://esbl.nhlbi.nih.gov/KTEA/). Protein abundance along the renal tubule for many commonly studied water and solute transport proteins and metabolic enzymes matched expectations from prior localization studies, demonstrating the overall reliability of the data. The site features a "correlated protein" function, which we used to identify cell type-specific transcription factors expressed along the renal tubule.
conclusionsWe identified and quantified proteins expressed in each of the 14 segments of rat kidney tubules and used the proteomic data that we obtained to create an online information resource, the Kidney Tubule Expression Atlas. This resource will allow users throughout the world to browse segment-specific protein expression data and download them for their own research.
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