ArticleCells2020
In Vitro Cytotoxicity Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells.
Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Impact of Nanoparticles on Male Fertility: What Do We Really Know? A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Cyclin D1/D2-CDK4 Drives Cell Migration by Orchestrating Cytoskeletal Dynamics Through a TGFβ-FAK-Rac1 Axis.International journal of molecular sciences · 2026Article
- Cardiac, reproductive, and brain toxicity of zinc oxide nanoparticles in male rats: Focus on morphological alterations and oxidative stress markers.Biochemistry and biophysics reports · 2025Article
- Effects of Surface Treatments on Innovative Additively Manufactured Scaffolds: Implications for Biocompatibility in Bone Tissue Engineering.Life (Basel, Switzerland) · 2025Article
- The dual effects of nanomaterials on sperm and seminal fluid oxidative stress.Materials today. Bio · 2025Review
- Article
- Zinc Oxide Nanoparticles Induced Testicular Toxicity Through Inflammation and Reducing Testosterone and Cell Viability in Adult Male Rats.Biological trace element research · 2025Article
- Strontium nitrate-dopped zinc oxide-loaded alginate gels with gentamicin for improved wound healing.Journal of materials science. Materials in medicine · 2025Article
- Advances in Nanomedicine: Transforming Diagnostic Imaging with Novel Contrast Agents.Current drug metabolism · 2025Review
- ρ-Coumaric acid-zinc oxide nanoparticles improve post-thaw quality of goat spermatozoa and developmental competence of fertilized oocytes in vitro.Scientific reports · 2024Article
- Antioxidant-related enzymes and peptides as biomarkers of metallic nanoparticles (eco)toxicity in the aquatic environment.Chemosphere · 2024Review
- ZnO NPs induce miR-342-5p mediated ferroptosis of spermatocytes through the NF-κB pathway in mice.Journal of nanobiotechnology · 2024Article
- Mesenchymal stem cells osteogenic differentiation by ZnO nanoparticles and polyurethane bimodal foam nanocomposites.Cell and tissue banking · 2024Article
- Nanoparticles-induced potential toxicity on human health: Applications, toxicity mechanisms, and evaluation models.MedComm · 2023Review
- Antibacterial Activity of Nanostructured Zinc Oxide Tetrapods.International journal of molecular sciences · 2023Article
- Methotrexate-conjugated zinc oxide nanoparticles exert a substantially improved cytotoxic effect on lung cancer cells by inducing apoptosis.Frontiers in pharmacology · 2023Article
- Different Strategies to Attenuate the Toxic Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells.Nanomaterials (Basel, Switzerland) · 2022Article
- Antimicrobial Perspectives of Active SiOPharmaceutics · 2022Article
- Exposure to zinc oxide nanoparticles affects testicular structure, reproductive development and spermatogenesis in parental and offspring male rats.Annals of translational medicine · 2022Article
- Nuclear Envelope Alterations in Myotonic Dystrophy Type 1 Patient-Derived Fibroblasts.International journal of molecular sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc Oxide Nanoparticles (ZnO NPs) are a type of metal oxide nanoparticle with an extensive use in biomedicine. Several studies have focused on the biosafety of ZnO NPs, since their size and surface area favor entrance and accumulation in the body, which can induce toxic effects. In previous studies, ZnO NPs have been identified as a dose- and time-dependent cytotoxic inducer in testis and male germ cells. However, the consequences for the first cell stage of spermatogenesis, spermatogonia, have never been evaluated. Therefore, the aim of the present work is to evaluate in vitro the cytotoxic effects of ZnO NPs in spermatogonia cells, focusing on changes in cytoskeleton and nucleoskeleton. For that purpose, GC-1 cell line derived from mouse testes was selected as a model of spermatogenesis. These cells were treated with different doses of ZnO NPs for 6 h and 12 h. The impact of GC-1 cells exposure to ZnO NPs on cell viability, cell damage, and cytoskeleton and nucleoskeleton dynamics was assessed. Our results clearly indicate that higher concentrations of ZnO NPs have a cytotoxic effect in GC-1 cells, leading to an increase of intracellular Reactive Oxygen Species (ROS) levels, DNA damage, cytoskeleton and nucleoskeleton dynamics alterations, and consequently cell death. In conclusion, it is here reported for the first time that ZnO NPs induce cytotoxic effects, including changes in cytoskeleton and nucleoskeleton in mouse spermatogonia cells, which may compromise the progression of spermatogenesis in a time- and dose-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.