ArticleAnnals of translational medicine2020
Rat lung transplantation model: modifications of the cuff technique.
Article in Annals of translational medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 25 citations in OpenAlex.
- Preconditioned endothelialized grafts reduce stenosis and improve vascular remodeling in a rat pulmonary artery model.Acta biomaterialia · 2026Article
- An integrated multiomic single-vesicle atlas delineates graft exosome heterogeneity in chronic lung allograft dysfunction.Signal transduction and targeted therapy · 2026Article
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- Refined cuff technique minimizes surgical complexity in rat lung transplantation and improves outcomes.Scientific reports · 2025Article
- An optimized perfusate for enhanced rat ex vivo lung perfusion and lung transplant models.Animal models and experimental medicine · 2025Article
- Activation of Nrf2 pathway by 4-Octyl itaconate enhances donor lung function in cold preservation settings.Respiratory research · 2025Article
- A novel rat lung transplantation model using venoarterial extracorporeal membrane oxygenation support.JTCVS techniques · 2024Article
- Hyperacute experimental model of rat lung transplantation using a coronary shunt cannula.World journal of transplantation · 2024Article
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- Disulfiram, an Anti-alcoholic Drug, Targets Macrophages and Attenuates Acute Rejection in Rat Lung Allografts.Transplant international : official journal of the European Society for Organ Transplantation · 2024Article
- Metformin attenuates chronic lung allograft dysfunction: evidence in rat models.Respiratory research · 2023Article
- Identification of cuproptosis-related biomarkers and analysis of immune infiltration in allograft lung ischemia-reperfusion injury.Frontiers in molecular biosciences · 2023Article
- Right lung transplantation with a left-to-right inverted anastomosis in a rat model.JTCVS open · 2022Article
- Improvement of surgical techniques for orthotopic single lung transplantation in rats.Annals of translational medicine · 2022Article
- A Comprehensive Review on the Surgical Aspect of Lung Transplant Models in Mice and Rats.Cells · 2022Review
- A Rat Lung Transplantation Model of Warm Ischemia/Reperfusion Injury: Optimizations to Improve Outcomes.Journal of visualized experiments : JoVE · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough the cuff technique in rat lung transplantation (LTx) has a long history, it remains technically challenging. We have developed key tricks and modifications in the devices and the cuff technique that optimize the rat LTx model to achieve successful operations during a short learning period.
methodsAltogether, 180 consecutive rats underwent orthotopic left LTx performed by a single surgeon using our modified devices and procedures. Allogeneic and syngeneic transplantation were performed using Lewis rats as recipients and Brown Norway and Lewis rats as donors. Allogeneic recipients were treated with cyclosporine during the first week. Recipients were sacrificed at various time points after ≥2 weeks.
resultsA special cuff-preparation plate was created using a petri dish and two foam blocks. This modified plate stabilizes the preparation and prevents donor lung compression. A "┴"-shaped incision was carved into the front wall of the pulmonary artery (PA) using micro-scissors. "V"-shaped incisions were made from the inferior-to-superior branches of the pulmonary vein (PV) and bronchus. A "pendulum model" was applied at implantation to make the hilar anastomosis tension-free and technically easier to perform. There were no intraoperative complications. Ten rats (5.6%) experienced partial or full pulmonary atelectasis. Five deaths (2.8%) due to pleural effusion occurred during the follow-up period. The operative times for heart-lung block retrieval, cuff preparation, cold ischemia, warm ischemia, and total procedure time were 8.4±0.8, 11.6±1.5, 25.1±2.2, 8.1±1.2, and 46.7±2.8 min, respectively.
conclusionsThe key tricks and improvements we made in the cuff technique for rat LTx provided the advantages of expeditiousness, a low complication rate, and a high success rate.
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