ArticleNature communications2020
A drug-inducible sex-separation technique for insects.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Generating cisgenic sexing strains in insect pests.Communications biology · 2026Article
- A Fluorescent Sex-Sorting Technique for Insects with the Demonstration inGEN biotechnology · 2024Article
- Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata.Scientific reports · 2023Article
- Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster.Current protocols · 2023Article
- Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.Current protocols · 2023Article
- Selective targeting of biting females to control mosquito-borne infectious diseases.Trends in parasitology · 2022Review
- Natural and Engineered Sex Ratio Distortion in Insects.Frontiers in ecology and evolution · 2022Article
- Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive.Nature communications · 2021Article
- Temperature-Inducible Precision-Guided Sterile Insect Technique.The CRISPR journal · 2021Article
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- Determining effective drug concentrations for selection and counterselection genetics inSTAR protocols · 2021Article
- Multiplexed drug-based selection and counterselection genetic manipulations in Drosophila.Cell reports · 2021Article
- Review
- A day in the life of a mosquito insectary team: pushing for solutions to mosquito-borne diseases.Lab animal · 2020Article
- Advances and challenges in genetic technologies to produce single-sex litters.PLoS genetics · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Here, we describe a drug-inducible genetic system for insect sex-separation that demonstrates proof-of-principle for positive sex selection in D. melanogaster. The system exploits the toxicity of commonly used broad-spectrum antibiotics geneticin and puromycin to kill the non-rescued sex. Sex-specific rescue is achieved by inserting sex-specific introns into the coding sequences of antibiotic-resistance genes. When raised on geneticin-supplemented food, the sex-sorter line establishes 100% positive selection for female progeny, while the food supplemented with puromycin positively selects 100% male progeny. Since the described system exploits conserved sex-specific splicing mechanisms and reagents, it has the potential to be adaptable to other insect species of medical and agricultural importance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.