ArticleIn vivo (Athens, Greece)
Diagnostic Algorithm in Hirschsprung's Disease: Focus on Immunohistochemistry Markers.
Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Clinicopathologic features of allied disorders of Hirschsprung disease and status update.World journal of gastrointestinal surgery · 2025Review
- The use of ultra-high frequency ultrasound in identifying aganglionosis in Hirschsprung's disease.Scientific reports · 2025Article
- Histopathologic Concerns and Diagnostic Challenges in Hirschsprung's Disease: An Eastern European Single-Center Observational Study.Life (Basel, Switzerland) · 2025Article
- Peripherin, A New Promising Biomarker in Neurological Disorders.The European journal of neuroscience · 2025Review
- Full-Thickness Rectal Biopsy in Children Suspected of Having Hirschsprung's Disease: The Inconclusive Biopsy.Children (Basel, Switzerland) · 2023Article
- Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung's Disease-Correlation and Agreement Analyses of Histoanatomy.Diagnostics (Basel, Switzerland) · 2023Article
- Histopathological dimensions differ between aganglionic and ganglionic bowel wall in children with Hirschsprung's disease.BMC pediatrics · 2022Observational
- Role of the Intermediate Filament Protein Peripherin in Health and Disease.International journal of molecular sciences · 2022Review
- Challenges in the diagnosis of intestinal neuronal dysplasia type B: A look beyond the number of ganglion cells.World journal of gastroenterology · 2021Review
- Clinical Spectrum and Outcomes of Neonatal Necrotizing Enterocolitis.In vivo (Athens, Greece)Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimHirschsprung disease (HD) is caused by the congenital absence of ganglion cells in the distal bowel (aganglionosis). Rectal biopsy is considered important for its diagnosis. The aim of this study was to apply immunohistochemical staining using a minimal set of antibodies and develop an algorithm that will assist in the diagnosis of HD. PATIENTS AND
methodsRectal or colonic biopsies were performed in patients with HD (n=26) or patients treated for other bowel diseases (n=34). Immunohistochemical staining was performed for MAP1b, peripherin, S-100, calretinin, NSE, bcl-2 and CD56 proteins.
resultsStaining for CD56, S-100, peripherin and calretinin facilitated the identification of ganglion cells. The use of CD56 and S-100 antibodies together resulted in the highest rate of ganglion cell staining intensity (94%).
conclusionWe propose a practical diagnostic algorithm with the application of CD56 and S-100 antibodies that can be used in clinical practice in children suspected of Hirschsprung's disease.
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