Evidence map›Paper›PMID 32353766›Full record

ArticleiScience2020

Injectable Therapeutic Organoids Using Sacrificial Hydrogels.

Ninna S Rossen, Priya N Anandakumaran, Rafael Zur Nieden, Kahmun Lo, Wenjie Luo, Christian Park, Chuqiao Huyan, Qinyouen Fu, Ziwei Song, Rajinder P Singh-Moon and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 2 countries.

Ninna S RossenDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA; Biotech Research & Innovation Centre, University of Copenhagen, University of Copenhagen, Ole MaaløesVej 5, 2200 Copenhagen N, Denmark; Department of Radiation Oncology, Stanford University, 269 Campus Drive, Palo Alto, CA 94305, USA.
Priya N AnandakumaranDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Rafael Zur NiedenDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Kahmun LoDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Wenjie LuoDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Christian ParkDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Chuqiao HuyanDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Qinyouen FuDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Ziwei SongDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Rajinder P Singh-MoonDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Janice ChungDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Jennifer E GoldenbergDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Nirali SampatDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Tetsuhiro HarimotoDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Danielle R BajakianDepartment of Surgery - Division of Vascular Surgery and Endovascular Interventions, Columbia University Medical Center, Herbert Irving Pavilion, 161 Fort Washington Avenue, New York, NY 10032, USA.
Brian M GilletteDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA.
Samuel K SiaDepartment of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA. Electronic address: ss2735@columbia.edu.
Columbia University · USColumbia University Irving Medical Center · USUniversity of Copenhagen · DK

Funding

New techniques for microfabricated vascularized 3D tissuesR01HL095477 · NHLBI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI SIA, SAMUEL K · 2010 to 2014
$1.9M
Injectable microtissues to promote rapid host re-perfusion of ischemic tissuesR01HL141935 · NHLBI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI SIA, SAMUEL K · 2018 to 2021
$1.6M
NHLBI NIH HHS R01 HL095477NHLBI NIH HHS R01 HL141935
6 · The paper itself

Abstract

Organoids are becoming widespread in drug-screening technologies but have been used sparingly for cell therapy as current approaches for producing self-organized cell clusters lack scalability or reproducibility in size and cellular organization. We introduce a method of using hydrogels as sacrificial scaffolds, which allow cells to form self-organized clusters followed by gentle release, resulting in highly reproducible multicellular structures on a large scale. We demonstrated this strategy for endothelial cells and mesenchymal stem cells to self-organize into blood-vessel units, which were injected into mice, and rapidly formed perfusing vasculature. Moreover, in a mouse model of peripheral artery disease, intramuscular injections of blood-vessel units resulted in rapid restoration of vascular perfusion within seven days. As cell therapy transforms into a new class of therapeutic modality, this simple method-by making use of the dynamic nature of hydrogels-could offer high yields of self-organized multicellular aggregates with reproducible sizes and cellular architectures.

Indexed as

BioelectronicsBiomaterialsBiotechnology

Identifiers

PMID32353766
PMCPMC7191221
OpenAlexW3015536249

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.