ArticleeLife2020
Effects of fluorescent glutamate indicators on neurotransmitter diffusion and uptake.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 82 citations in OpenAlex.
- Illuminating the Future of Catecholamine Detection.Journal of neurochemistry · 2026Review
- Ratiometric iGluSnFr imaging to assess tonic glutamate in the cerebral cortex.bioRxiv : the preprint server for biology · 2026Article
- The multiple scales of astrocytic functional units.Nature neuroscience · 2026Review
- Bipolar cell networks underlying steady-state intensity encoding in intrinsically photosensitive retinal ganglion cells.iScience · 2026Article
- A heterogeneous population code at the first synapse of vision.Nature communications · 2026Article
- Metabolic interactions in the brain: the crucial roles of neurons, astrocytes, and microglia in health and disease.Frontiers in neuroscience · 2026Review
- Atypical plume-like events contribute to glutamate accumulation in metabolic stress conditions.iScience · 2025Article
- Glutamate uptake is transiently compromised in the perilesional cortex following controlled cortical impact.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- Substance P and dopamine form a "push-pull" system that diurnally regulates retinal gain.Current biology : CB · 2024Article
- Observation of Brownian elastohydrodynamic forces acting on confined soft colloids.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Brain energy metabolism: A roadmap for future research.Journal of neurochemistry · 2024Review
- Increased presynaptic excitability in a migraine with aura mutation.Brain : a journal of neurology · 2024Article
- Optical measurement of glutamate release robustly reports short-term plasticity at a fast central synapse.Frontiers in molecular neuroscience · 2024Article
- Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells.eLife · 2023Article
- Functional maturation of the rod bipolar to AII-amacrine cell ribbon synapse in the mouse retina.Cell reports · 2023Article
- Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells.bioRxiv : the preprint server for biology · 2023Article
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- Volume-transmitted GABA waves pace epileptiform rhythms in the hippocampal network.Current biology : CB · 2023Article
- Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo.Journal of neurochemistry · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Genetically encoded fluorescent glutamate indicators (iGluSnFRs) enable neurotransmitter release and diffusion to be visualized in intact tissue. Synaptic iGluSnFR signal time courses vary widely depending on experimental conditions, often lasting 10-100 times longer than the extracellular lifetime of synaptically released glutamate estimated with uptake measurements. iGluSnFR signals typically also decay much more slowly than the unbinding kinetics of the indicator. To resolve these discrepancies, here we have modeled synaptic glutamate diffusion, uptake and iGluSnFR activation to identify factors influencing iGluSnFR signal waveforms. Simulations suggested that iGluSnFR competes with transporters to bind synaptically released glutamate, delaying glutamate uptake. Accordingly, synaptic transporter currents recorded from iGluSnFR-expressing astrocytes in mouse cortex were slower than those in control astrocytes. Simulations also suggested that iGluSnFR reduces free glutamate levels in extrasynaptic spaces, likely limiting extrasynaptic receptor activation. iGluSnFR and lower affinity variants, nonetheless, provide linear indications of vesicle release, underscoring their value for optical quantal analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.