ArticleApplications in plant sciences2020
A basic ddRADseq two-enzyme protocol performs well with herbarium and silica-dried tissues across four genera.
Article in Applications in plant sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- Next-Generation Genotyping: Innovations Driving Plant Genomic Improvement.Life (Basel, Switzerland) · 2026Review
- Evaluation of DNA yield from various tissue and sampling sources for use in single nucleotide polymorphism panels.Scientific reports · 2024Article
- Retrieval of long DNA reads from herbarium specimens.AoB PLANTS · 2023Review
- A Foundational Population Genetics Investigation of the Sexual Systems ofPlants (Basel, Switzerland) · 2023Article
- Diversity analyses in two ornamental and large-genomeFrontiers in plant science · 2023Article
- Tracking population genetic signatures of local extinction with herbarium specimens.Annals of botany · 2022Article
- SNP markers reveal relationships between fruit paternity, fruit quality and distance from a cross-pollen source in avocado orchards.Scientific reports · 2021Article
- On the potential of Angiosperms353 for population genomic studies.Applications in plant sciences · 2021Article
- The Genetic Basis of Tomato Aroma.Genes · 2021Review
- High-throughput methods for efficiently building massive phylogenies from natural history collections.Applications in plant sciences · 2021Article
- A basic ddRADseq two-enzyme protocol performs well with herbarium and silica-dried tissues across four genera.Applications in plant sciences · 2020Article
- A comparison of freezer-stored DNA and herbarium tissue samples for chloroplast assembly and genome skimming.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
premiseThe ability to sequence genome-scale data from herbarium specimens would allow for the economical development of data sets with broad taxonomic and geographic sampling that would otherwise not be possible. Here, we evaluate the utility of a basic double-digest restriction site-associated DNA sequencing (ddRADseq) protocol using DNAs from four genera extracted from both silica-dried and herbarium tissue.
methodsDNAs from
resultsAlthough taxon, preservation method, and specimen age affected data recovery, large phylogenetically informative data sets were obtained from the majority of samples. DISCUSSION: These results suggest that herbarium samples can be incorporated into ddRADseq project designs, and that specimen age can be used as a rapid on-site guide for sample choice. The detailed protocol we provide will allow users to pursue herbarium-based ddRADseq projects that minimize the expenses associated with fieldwork and sample evaluation.
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Registered trials
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