ArticleFrontiers in genetics2020
Single-Cell Transcriptomes Reveal a Complex Cellular Landscape in the Middle Ear and Differential Capacities for Acute Response to Infection.
Article in Frontiers in genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Type I interferon signaling in hematopoietic cells impairs neutrophil antibacterial function in the middle ear during viral co-infection.Cell reports. Medicine · 2026Article
- Amelogenin Phosphorylation Affects Key Regulatory Genes in the Enamel Organ.Calcified tissue international · 2026Review
- Systems perspectives on pediatric otitis media: environmental exposures, genetic susceptibility, and biomarker-guided interventions.Frontiers in pediatrics · 2026Review
- Rare and low-frequency variants in families with otitis media.Journal of molecular medicine (Berlin, Germany) · 2025Article
- A NovelInternational journal of molecular sciences · 2025Article
- Single-cell RNA sequencing data analysis of the inner ear in gentamicin-treated mice via intraperitoneal injection.Open medicine (Warsaw, Poland) · 2025Article
- Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats.Regenerative therapy · 2022Article
- Multimerin-1 and cancer: a review.Bioscience reports · 2022Review
- Novel candidate genes for cholesteatoma in chronic otitis media.Frontiers in genetics · 2022Article
- The ECRG4 cleavage product augurin binds the endotoxin receptor and influences the innate immune response during otitis media.Frontiers in genetics · 2022Article
- Trans-cortical vessels in the mouse temporal bulla bone are a means to recruit myeloid cells in chronic otitis media and limit peripheral leukogram changes.Frontiers in genetics · 2022Article
- HB-EGF Plays a Pivotal Role in Mucosal Hyperplasia During Otitis Media Induced by a Viral Analog.Frontiers in cellular and infection microbiology · 2022Article
- Immunomodulation as a Protective Strategy in Chronic Otitis Media.Frontiers in cellular and infection microbiology · 2022Article
- Essential Role of the Innate Immune Adaptor RIP2 in the Response to Otitis Media.Frontiers in genetics · 2022Article
- Middle Ear "Adenoma": a Neuroendocrine Tumor with Predominant L Cell Differentiation.Endocrine pathology · 2021Article
- The role of CDHR3 in susceptibility to otitis media.Journal of molecular medicine (Berlin, Germany) · 2021Article
- The transcriptional landscape of the cultured murine middle ear epithelium in vitro.Biology open · 2021Article
- TheFrontiers in cellular and infection microbiology · 2021Article
- Leukotriene B4 Is a Major Determinant of Leukocyte Recruitment During Otitis Media.Frontiers in cellular and infection microbiology · 2021Article
- Global Pattern of CD8Frontiers in pharmacology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Single-cell transcriptomics was used to profile cells of the normal murine middle ear. Clustering analysis of 6770 transcriptomes identified 17 cell clusters corresponding to distinct cell types: five epithelial, three stromal, three lymphocyte, two monocyte, two endothelial, one pericyte and one melanocyte cluster. Within some clusters, cell subtypes were identified. While many corresponded to those cell types known from prior studies, several novel types or subtypes were noted. The results indicate unexpected cellular diversity within the resting middle ear mucosa. The resolution of uncomplicated, acute, otitis media is too rapid for cognate immunity to play a major role. Thus innate immunity is likely responsible for normal recovery from middle ear infection. The need for rapid response to pathogens suggests that innate immune genes may be constitutively expressed by middle ear cells. We therefore assessed expression of innate immune genes across all cell types, to evaluate potential for rapid responses to middle ear infection. Resident monocytes/macrophages expressed the most such genes, including pathogen receptors, cytokines, chemokines and chemokine receptors. Other cell types displayed distinct innate immune gene profiles. Epithelial cells preferentially expressed pathogen receptors, bactericidal peptides and mucins. Stromal and endothelial cells expressed pathogen receptors. Pericytes expressed pro-inflammatory cytokines. Lymphocytes expressed chemokine receptors and antimicrobials. The results suggest that tissue monocytes, including macrophages, are the master regulators of the immediate middle ear response to infection, but that virtually all cell types act in concert to mount a defense against pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.