Evidence map›Paper›PMID 32351208›Full record

ArticleParasite (Paris, France)2020

Development of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals.

Basma Ouarti, Maureen Laroche, Souad Righi, Mohamed Nadir Meguini, Ahmed Benakhla, Didier Raoult, Philippe Parola

Abstract read
In one paragraph

Article in Parasite (Paris, France), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

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  15. Identification of AdultMicroorganisms · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Basma OuartiAix Marseille Univ., IRD, AP-HM, SSA, VITROME, 13005 Marseille, France - IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.
Maureen LarocheAix Marseille Univ., IRD, AP-HM, SSA, VITROME, 13005 Marseille, France - IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.
Souad RighiUniversité Chadli Bendjdid, Département des sciences Vétérinaire, 36000 El Tarf, Algeria.
Mohamed Nadir MeguiniUniversité Chadli Bendjdid, Département des sciences Vétérinaire, 36000 El Tarf, Algeria - Institut des Sciences Vétérinaire et Agronomiques, Université Mohamed Cherif Messaadia, 41000 Souk-Ahras, Algeria.
Ahmed BenakhlaUniversité Chadli Bendjdid, Département des sciences Vétérinaire, 36000 El Tarf, Algeria.
Didier RaoultIHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France - Aix Marseille Univ., IRD, AP-HM, MEPHI, 13005 Marseille, France.
Philippe ParolaAix Marseille Univ., IRD, AP-HM, SSA, VITROME, 13005 Marseille, France - IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.

Indexed as

AlgeriaAnimalsAnopluraCattleChickensGoatsLice InfestationsLivestockPhthirapteraPoultryProteomicsReproducibility of ResultsSheepSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnopluraLiceMALDI-TOF MSMallophagaPhthiraptera

Identifiers

PMID32351208
PMCPMC7191974

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.