ArticleThe EMBO journal2020
Structural basis for loading and inhibition of a bacterial T6SS phospholipase effector by the VgrG spike.
Article in The EMBO journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 46 citations in OpenAlex.
- From gut colonization to recurrence:Gut microbes · 2026Review
- Contributions of conserved and species-specific CagX (VirB9) domains to the assembly and function of theInfection and immunity · 2026Article
- Molecular basis of type VI secretion system effector loading.Nature microbiology · 2026Article
- Tvp complexes support formation of the VgrG-PAAR spike during Type VI secretion system assembly.The EMBO journal · 2026Article
- A T4SS effector directly disarms competing antibacterial T6SSs by hijacking their conserved spike component.Science China. Life sciences · 2026Article
- The type VI secretion system and associated effector proteins.Nature reviews. Microbiology · 2026Review
- Molecular strategies ofFrontiers in cellular and infection microbiology · 2026Review
- Integrating natural language processing and genome analysis enables accurate bacterial phenotype prediction.NAR genomics and bioinformatics · 2025Article
- Delivery determinants of anmBio · 2025Article
- Specialized killing across the domains of life by the type VI secretion systems of Pseudomonas aeruginosa.The Biochemical journal · 2025Review
- Thioredoxin 1 moonlights as a chaperone for an interbacterial ADP-ribosyltransferase toxin.Nature communications · 2024Article
- Structure of a Rhs effector clade domain provides mechanistic insights into type VI secretion system toxin delivery.Nature communications · 2024Article
- Identification of type VI secretion system effector-immunity pairs using structural bioinformatics.Molecular systems biology · 2024Article
- Role of Type VI secretion system in pathogenic remodeling of host gut microbiota during Aeromonas veronii infection.The ISME journal · 2024Article
- The type VI secretion system of Stenotrophomonas rhizophila CFBP13503 limits the transmission of Xanthomonas campestris pv. campestris 8004 from radish seeds to seedlings.Molecular plant pathology · 2024Article
- Genome wide analysis revealed conserved domains involved in the effector discrimination of bacterial type VI secretion system.Communications biology · 2023Article
- The RIX domain defines a class of polymorphic T6SS effectors and secreted adaptors.Nature communications · 2023Article
- Killing in the name of: T6SS structure and effector diversity.Microbiology (Reading, England) · 2023Review
- Diversity of the type VI secretion systems in theMicrobial genomics · 2023Article
- Activity and Crystal Structure of the Adherent-InvasiveInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
The bacterial type VI secretion system (T6SS) is a macromolecular machine that injects effectors into prokaryotic and eukaryotic cells. The mode of action of the T6SS is similar to contractile phages: the contraction of a sheath structure pushes a tube topped by a spike into target cells. Effectors are loaded onto the spike or confined into the tube. In enteroaggregative Escherichia coli, the Tle1 phospholipase binds the C-terminal extension of the VgrG trimeric spike. Here, we purify the VgrG-Tle1 complex and show that a VgrG trimer binds three Tle1 monomers and inhibits their activity. Using covalent cross-linking coupled to high-resolution mass spectrometry, we provide information on the sites of contact and further identify the requirement for a Tle1 N-terminal secretion sequence in complex formation. Finally, we report the 2.6-Å-resolution cryo-electron microscopy tri-dimensional structure of the (VgrG)
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.