ArticleMikrochimica acta2020
A fluorescent aptasensor for Staphylococcus aureus based on strand displacement amplification and self-assembled DNA hexagonal structure.
Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Advances in biosensor technologies for the detection of antimicrobial resistance inFrontiers in cellular and infection microbiology · 2025Review
- Review
- A enzyme-free fluorescence quenching sensor for amplified detection of kanamycin in milk based on competitive triggering strategies.RSC advances · 2024Article
- Rapid detection methods for foodborne pathogens based on nucleic acid amplification: Recent advances, remaining challenges, and possible opportunities.Food chemistry. Molecular sciences · 2023Review
- Toward Continuous Molecular Testing Using Gold-Coated Threads as Multi-Target Electrochemical Biosensors.Biosensors · 2023Article
- An Integrated Microfluidic Biosensing System Based on a Versatile Valve and Recombinase Polymerase Amplification for Rapid and Sensitive Detection ofBiosensors · 2023Article
- Multiple amplification-based fluorometric aptasensor for highly sensitive detection of Staphylococcus aureus.Applied microbiology and biotechnology · 2022Article
- Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer.Sensors (Basel, Switzerland) · 2022Article
- Recent Advances in Aptasensors For Rapid and Sensitive Detection ofFrontiers in bioengineering and biotechnology · 2022Review
- DNA Nanostructures in the Fight Against Infectious Diseases.Advanced nanobiomed research · 2021Article
- Review
- Recent Progress in the Identification of Aptamers Against Bacterial Origins and Their Diagnostic Applications.International journal of molecular sciences · 2020Review
- Recent Advances in Aptamer-Based Biosensors for Detection ofFrontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
A fluorescent aptasensor for Staphylococcus aureus (S. aureus) is designed, which takes advantage of strand displacement amplification (SDA) technology and unique self-assembled DNA hexagonal structure. In the presence of S. aureus, a partially complementary strand of S. aureus aptamer (cDNA) is competitively released from cDNA/aptamer duplex immobilized on magnetic beads due to the affinity of the aptamer for S. aureus. The addition of primer starts the SDA reaction. With the catalysis of Bsm DNA polymerase and Nb.bpu10I endonuclease, a large number of single-stranded DNA (ssDNA) is produced, which induces the opening of a hairpin probe and the subsequent self-assembly to form a hexagonal structure. The staining of the DNA hexagon with SYBR Green I excites the fluorescence signal, which is used for detection. The aptasensor exhibits a broad linear range from 7 to 7 × 10
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Registered trials
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