ArticleCommunications biology2020
An artificial chromosome ylAC enables efficient assembly of multiple genes in Yarrowia lipolytica for biomanufacturing.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- 2A peptides enable simplified discovery and heterologous production of fungal bioactive metabolites in Yarrowia lipolytica.Applied microbiology and biotechnology · 2026Article
- In Vivo DNA Assembly inACS synthetic biology · 2025Article
- Review
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- Article
- Progress in the Metabolic Engineering ofBiodesign research · 2024Review
- A supernumerary synthetic chromosome in Komagataella phaffii as a repository for extraneous genetic material.Microbial cell factories · 2023Article
- Installing xylose assimilation and cellodextrin phosphorolysis pathways in obese Yarrowia lipolytica facilitates cost-effective lipid production from lignocellulosic hydrolysates.Biotechnology for biofuels and bioproducts · 2023Article
- New roles for Yarrowia lipolytica in molecules synthesis and biocontrol.Applied microbiology and biotechnology · 2022Review
- Efficient production of the β-ionone aroma compound from organic waste hydrolysates using an engineeredFrontiers in microbiology · 2022Article
- Synthetic Genomics From a Yeast Perspective.Frontiers in bioengineering and biotechnology · 2022Review
- Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering.Applied microbiology and biotechnology · 2021Article
- Review
- Yeast-Based Biosynthesis of Natural Products From Xylose.Frontiers in bioengineering and biotechnology · 2021Review
- Synthetic biology, systems biology, and metabolic engineering of Yarrowia lipolytica toward a sustainable biorefinery platform.Journal of industrial microbiology & biotechnology · 2020Review
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Authors and funding
7 authors.
Funding
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Abstract
The efficient use of the yeast Yarrowia lipolytica as a cell factory is hampered by the lack of powerful genetic engineering tools dedicated for the assembly of large DNA fragments and the robust expression of multiple genes. Here we describe the design and construction of artificial chromosomes (ylAC) that allow easy and efficient assembly of genes and chromosomal elements. We show that metabolic pathways can be rapidly constructed by various assembly of multiple genes in vivo into a complete, independent and linear supplementary chromosome with a yield over 90%. Additionally, our results reveal that ylAC can be genetically maintained over multiple generations either under selective conditions or, without selective pressure, using an essential gene as the selection marker. Overall, the ylACs reported herein are game-changing technology for Y. lipolytica, opening myriad possibilities, including enzyme screening, genome studies and the use of this yeast as a previous unutilized bio-manufacturing platform.
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