ArticleScientific reports2020
Minimally Invasive Cell-Free Human Embryo Aneuploidy Testing (miPGT-A) Utilizing Combined Spent Embryo Culture Medium and Blastocoel Fluid -Towards Development of a Clinical Assay.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04856696 (Clinical Application of Non-invasive Preimplantation Genetic Testing for Aneuploidies), which is not on this map. Cited by 29 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Clinical Application of Non-invasive Preimplantation Genetic Testing for Aneuploidies
Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.
- The diagnostic accuracy of preimplantation genetic testing (PGT) in assessing the genetic status of embryos: a systematic review and meta-analysis.Reproductive biology and endocrinology : RB&E · 2025Pooled it
- Evaluation of non-invasive gene detection in preimplantation embryos: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2023Pooled it
- A prospective study of non-invasive preimplantation genetic testing for Rh status, sex prediction and mtDNA copy number analysis using ddPCR on spent culture media.Experimental and therapeutic medicine · 2026Article
- Observational
- Genetic analysis of biopsy-related droplets in patients undergoing PGT-A and its potential application.Frontiers in endocrinology · 2026Article
- Application of non-invasive preimplantation genetic screening for aneuploidy through spent embryo culture media analysis at 48 and 54 hours after embryo cleavage.British journal of biomedical science · 2026Article
- MicroRNAs Secreted by the Embryo in Spent Culture Medium Can Regulate mRNAs Involved in Endometrial Receptivity, Embryo Attachment, and Invasion.International journal of molecular sciences · 2025Article
- The Role of Preconception Parental Health on Embryo Quality-Preliminary Results of a Prospective Study Using Non-Invasive Preimplantation Genetic Testing for Aneuploidy.Biomolecules · 2025Article
- Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Non-invasive preimplantation genetic testing for aneuploidy using cell-free DNA in blastocyst culture medium.Journal of assisted reproduction and genetics · 2025Article
- Non-Invasive Preimplantation Genetic Testing.Genes · 2025Review
- Double versus single blastocyst biopsy and vitrification in preimplantation genetic testing (PGT) cycles: protocol for a systematic review and meta-analysis of clinical and neonatal outcomes.Systematic reviews · 2025Article
- Non Invasive Preimplantation Testing for Aneuploidies in Assisted Reproduction: A SWOT Analysis.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Comparison of Non-Invasive and Minimally Invasive Preimplantation Genetic Testing for Aneuploidy Using Samples Derived from the Same Embryo Culture.Journal of clinical medicine · 2024Article
- Comparing the advantages, disadvantages and diagnostic power of different non-invasive pre-implantation genetic testing: A literature review.International journal of reproductive biomedicine · 2023Review
- microRNAs as A Biomarker to Predict Embryo Quality Assessment in In Vitro Fertilization.International journal of fertility & sterility · 2023Article
- The embryo non-invasive pre-implantation diagnosis era: how far are we?Animal reproduction · 2023Article
- An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo.Current genomics · 2022Review
- Majority of transferred mosaic embryos developed healthy live births revealed by a preclinical study using embryonic morphology assessment and noninvasive PGT-A on cell-free DNA in blastocoel fluid.Journal of assisted reproduction and genetics · 2022Article
- Development of an artificial intelligence model for predicting the likelihood of human embryo euploidy based on blastocyst images from multiple imaging systems during IVF.Human reproduction (Oxford, England) · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preimplantation genetic testing for aneuploidies (PGT-A) using trophectoderm (TE) biopsy samples is labour intensive, invasive, and subject to sampling bias. In this study, we report on the efficacy and factors affecting accuracy of a technique we pioneered for minimally invasive preimplantation genetic testing for aneuploidy (miPGT-A). Our technique uses cell-free embryonic DNA (cfeDNA) in spent embryo culture medium (SEM) combined with blastocoel fluid (BF) to increase the amount of assayable cfeDNA. We compared miPGT-A results (n = 145 embryos) with standard PGT-A analysis of the corresponding trophectoderm biopsy. We found that accuracy of miPGT was not related to blastocyst morphological grade. The overall concordance rate per sample for euploidy/aneuploidy status between miPGT-A and TE biopsy samples was 88/90 (97.8%), and was not different between good 47/48 (97.9%) and moderate/low quality blastocysts 41/42 (97.9%) (p > 0.05). Importantly, we also discovered that for cfeDNA analysis, the SurePlex whole genome amplification (WGA) kit can be utilized without an additional cell lysis/extraction DNA step; this efficiency likely reduces the risk of maternal contamination. Regarding origin of embryonic cfeDNA, the average amount of miPGT-A WGA-DNA we obtained from blastocysts with different morphological grades, as well as the size miPGT-A WGA-DNA fragments, suggest that it is unlikely that apoptosis and necrosis are only mechanisms of DNA release from the inner cell mass (ICM) and TE into BF and SEM.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.