Evidence map›Paper›PMID 32350359›Full record

ArticleScientific reports2020

Detection of Neorickettsia risticii, the agent of Potomac horse fever, in horses from Rio de Janeiro, Brazil.

Patrícia Gonzaga Paulino, Nádia Almosny, Renata Oliveira, Vanessa Viscardi, Ananda Müller, Andresa Guimarães, Cristiane Baldani, Claudia da Silva, Maristela Peckle, Carlos Massard and 1 more

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Genetic diversity and host-symbiont cophylogenetic analysis ofCurrent research in parasitology & vector-borne diseases · 2026
    Article
  3. Review
  4. Article
  5. Diversity ofCurrent research in parasitology & vector-borne diseases · 2024
    Article
  6. Neorickettsia sp. in coatis (Nasua nasua) in Brazil.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2023
    Article
  7. Improved molecular detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Patrícia Gonzaga PaulinoDepartment of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil. patgpaulino@gmail.com.
Nádia AlmosnyDepartment of Veterinary Clinic and Pathology, Federal Fluminense University, Niteroi, Brazil.
Renata OliveiraDepartment of Veterinary Clinic and Pathology, Federal Fluminense University, Niteroi, Brazil.
Vanessa ViscardiDepartment of Veterinary Clinic and Pathology, Federal Fluminense University, Niteroi, Brazil.
Ananda MüllerRoss University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Andresa GuimarãesDepartment of Veterinary Medicine and Surgery, Veterinary Institute, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil.ORCID http://orcid.org/0000-0002-4064-0710
Cristiane BaldaniDepartment of Veterinary Medicine and Surgery, Veterinary Institute, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil.
Claudia da SilvaDepartment of Animal Parasitology, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil.
Maristela PeckleDepartment of Animal Parasitology, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil.
Carlos MassardDepartment of Animal Parasitology, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil.
Huarrisson SantosDepartment of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro (UFRRJ), BR 465, Km 7, Seropedica, RJ, 23890000, Brazil.
Universidade Federal Rural do Rio de Janeiro · BRUniversidade Federal Fluminense · BRAustral University of Chile · CL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aims to report the presence of Neorickettsia risticii DNA in blood samples from naturally infected horses in Rio de Janeiro, provide clinicopathological findings related to the infection, and report the phylogenetic diversity of the 16S rDNA of N. risticii in order to evaluate its heterogeneity. Real-time quantitative polymerase chain reaction (qPCR) was performed to investigate the presence of N. risticii in samples collected from horses (n = 187). Five positive samples were found in the molecular screening. Hypoalbuminemia and high levels of creatine kinase and lactate dehydrogenase were the predominant findings in the biochemical analysis. The sequences were similar to those of N. risticii. Phylogenetic analysis revealed genotype segregation based on the geographical distribution in the N. risticii sequence clade. Dendrograms constructed with five hypervariable regions revealed that V4 distinguished Neorickettsia at the species level and produced a phylogeny that best represented the phylogeny obtained with the complete 16S rDNA sequence. This is the first report of N. risticii DNA in the blood of Brazilian horses based on sequences deposited in GenBank. Further studies are necessary to clarify the epidemiological chain of this vector-borne parasite in order to determine and establish appropriate preventive measures in the equine trading market.

Indexed as

Anaplasmataceae InfectionsHorse DiseasesHorsesPhylogenyAnimalsBrazilDNA, BacterialDNA, RibosomalNeorickettsia risticiiRNA, BacterialRNA, Ribosomal, 16SDNA, BacterialDNA, RibosomalRNA, BacterialRNA, Ribosomal, 16S

Identifiers

PMID32350359
PMCPMC7190851
OpenAlexW3022489758

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.