ArticleScientific reports2020
Selection of viral variants during persistent infection of insectivorous bat cells with Middle East respiratory syndrome coronavirus.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Diverse hosts, diverse immune systems: Evolutionary variation in bat immunology.Annals of the New York Academy of Sciences · 2025Review
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- Studying bats using a One Health lens: bridging the gap between bat virology and disease ecology.Journal of virology · 2024Review
- Contact-number-driven virus evolution: A multi-level modeling framework for the evolution of acute or persistent RNA virus infection.PLoS computational biology · 2023Article
- Contribution to pathogenesis of accessory proteins of deadly human coronaviruses.Frontiers in cellular and infection microbiology · 2023Review
- Long Covid: where we stand and challenges ahead.Cell death and differentiation · 2022Review
- Review
- Testing bats in rehabilitation for SARS-CoV-2 before release into the wild.Conservation science and practice · 2022Article
- Ecology, evolution and spillover of coronaviruses from bats.Nature reviews. Microbiology · 2022Review
- The risk from SARS-CoV-2 to bat species in england and mitigation options for conservation field workers.Transboundary and emerging diseases · 2022Article
- Reduced IFN-ß inhibitory activity of Lagos bat virus phosphoproteins in human compared to Eidolon helvum bat cells.PloS one · 2022Article
- Cross-species transmission, evolution and zoonotic potential of coronaviruses.Frontiers in cellular and infection microbiology · 2022Review
- COVID-19: comprehensive review on mutations and current vaccines.Archives of microbiology · 2021Review
- Bats and viruses: a death-defying friendship.Virusdisease · 2021Review
- Experimental and natural evidence of SARS-CoV-2-infection-induced activation of type I interferon responses.iScience · 2021Article
- Coronavirus Disease 2019: Clinics, Treatment, and Prevention.Frontiers in microbiology · 2021Review
- Biosensors for Point Mutation Detection.Frontiers in bioengineering and biotechnology · 2021Article
- The Coronaviruses of Animals and Birds: Their Zoonosis, Vaccines, and Models for SARS-CoV and SARS-CoV2.Frontiers in veterinary science · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coronaviruses that cause severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) are speculated to have originated in bats. The mechanisms by which these viruses are maintained in individuals or populations of reservoir bats remain an enigma. Mathematical models have predicted long-term persistent infection with low levels of periodic shedding as a likely route for virus maintenance and spillover from bats. In this study, we tested the hypothesis that bat cells and MERS coronavirus (CoV) can co-exist in vitro. To test our hypothesis, we established a long-term coronavirus infection model of bat cells that are persistently infected with MERS-CoV. We infected cells from Eptesicus fuscus with MERS-CoV and maintained them in culture for at least 126 days. We characterized the persistently infected cells by detecting virus particles, protein and transcripts. Basal levels of type I interferon in the long-term infected bat cells were higher, relative to uninfected cells, and disrupting the interferon response in persistently infected bat cells increased virus replication. By sequencing the whole genome of MERS-CoV from persistently infected bat cells, we identified that bat cells repeatedly selected for viral variants that contained mutations in the viral open reading frame 5 (ORF5) protein. Furthermore, bat cells that were persistently infected with ΔORF5 MERS-CoV were resistant to superinfection by wildtype virus, likely due to reduced levels of the virus receptor, dipeptidyl peptidase 4 (DPP4) and higher basal levels of interferon in these cells. In summary, our study provides evidence for a model of coronavirus persistence in bats, along with the establishment of a unique persistently infected cell culture model to study MERS-CoV-bat interactions.
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