ArticleNPJ biofilms and microbiomes2020
Network-directed efficient isolation of previously uncultivated Chloroflexi and related bacteria in hot spring microbial mats.
Article in NPJ biofilms and microbiomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed.
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- Mining Microbial Dark Matter: Advanced Cultivation Techniques for Bioactive Compound Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Genomic insights into the mangrove-associated cultivable bacteria and their ecological function.iScience · 2025Article
- Defining the cultured and uncultured bacterial fractions in Cannabis seeds.Environmental microbiome · 2025Article
- A metagenomic perspective on the microbial prokaryotic genome census.Science advances · 2025Article
- Novel diversity of Anaerolineae and Tepidiformia recovered from metagenomes of thermal microbial mats in Costa Rica.Frontiers in microbiology · 2025Article
- A salt-tolerant growth-promoting phyllosphere microbial combination from mangrove plants and its mechanism for promoting salt tolerance in rice.Microbiome · 2024Article
- Mining microbial and metabolic dark matter in extreme environments: a roadmap for harnessing the power of multi-omics data.Advanced biotechnology · 2024Review
- Cultivation of Diverse Novel Marine Bacteria from Deep Ocean Sediment Using Spent Culture Supernatant of Ca. Bathyarchaeia Enrichment.Journal of microbiology (Seoul, Korea) · 2024Article
- Article
- Culturomics- and metagenomics-based insights into the soil microbiome preservation and application for sustainable agriculture.Frontiers in microbiology · 2024Review
- Water masses influence the variation of microbial communities in the Yangtze River Estuary and its adjacent waters.Frontiers in microbiology · 2024Article
- Bacterial diversity in water from Xifeng Hot Spring in China.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Article
- Uncultured Microorganisms and Their Functions in the Fermentation Systems of Traditional Chinese Fermented Foods.Foods (Basel, Switzerland) · 2023Review
- Microbial Interaction is Among the Key Factors for Isolation of Previous Uncultured Microbes.Journal of microbiology (Seoul, Korea) · 2023Review
- Computational design of CRISPR guide RNAs to enable strain-specific control of microbial consortia.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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13 authors.
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Abstract
The perplexity of the complex multispecies community interactions is one of the many reasons why majority of the microorganisms are still uncultivated. We analyzed the entire co-occurrence networks between the OTUs of Tibet and Yunnan hot spring samples, and found that less abundant OTUs such as genus Tepidimonas (relative abundant <1%) had high-degree centricity (key nodes), while dominant OTUs particularly genus Chloroflexus (relative abundant, 13.9%) formed the peripheral vertexes. A preliminary growth-promotion assay determined that Tepidimonas sp. strain SYSU G00190W enhanced the growth of Chloroflexus sp. SYSU G00190R. Exploiting this result, an ameliorated isolation medium containing 10% spent-culture supernatant of Tepidimonas sp. strain SYSU G00190W was prepared for targeted isolation of Chloroflexi in the Tibet and Yunnan hot spring samples. 16S rRNA gene fingerprinting characterized majority of the colonies isolated from these media as previously uncultivated Chloroflexi, of which 36 are potential novel species (16S rRNA sequence identity <98.5%). Metabolomes studies indicated that the spent-culture supernatant comprises several low-molecular-weight organic substrates that can be utilized as potential nutrients for the growth of these bacteria. These findings suggested that limited knowledge on the interaction of microbes provide threshold to traditional isolation method.
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