ArticleProceedings of the National Academy of Sciences of the United States of America2020
All-electrical monitoring of bacterial antibiotic susceptibility in a microfluidic device.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 86 citations in OpenAlex.
- Electrochemical Biosensing for Antibiotic-Resistant Bacteria: Advances, Challenges, and Future Directions.Micromachines · 2025Review
- Rapid antimicrobial susceptibility testing using carbon screen printed electrodes in a microfluidic device.Scientific reports · 2025Article
- Advancements in AI-driven drug sensitivity testing research.Frontiers in cellular and infection microbiology · 2025Review
- Single-Cell Microfluidics: A Primer for Microbiologists.The journal of physical chemistry. B · 2024Review
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Automated and miniaturized screening of antibiotic combinationsActa pharmaceutica Sinica. B · 2024Article
- Cost-Efficient Micro-Well Array-Based Colorimetric Antibiotic Susceptibility Testing (MacAST) for Bacteria from Culture or Community.Biosensors · 2023Article
- Projection Micro-Stereolithography to Manufacture a Biocompatible Micro-Optofluidic Device for Cell Concentration Monitoring.Polymers · 2023Article
- Accurate Prediction of Antimicrobial Susceptibility for Point-of-Care Testing of Urine in Less than 90 Minutes via iPRISM Cassettes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenicSensors & diagnostics · 2023Article
- Rapid Phenotypic Antimicrobial Susceptibility Testing Using a Coulter Counter and Proliferation Rate Discrepancy.ACS omega · 2023Article
- Electrical Broth Micro-Dilution for Rapid Antibiotic Resistance Testing.ACS sensors · 2023Article
- Advances in the screening of antimicrobial compounds using electrochemical biosensors: is there room for nanomaterials?Analytical and bioanalytical chemistry · 2023Review
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Early appropriate diagnostics and treatment of MDR Gram-negative infections.JAC-antimicrobial resistance · 2022Review
- Measurement of the low-frequency charge noise of bacteria.Physical review. E · 2022Article
- Single-Cell Motility Rapidly Quantifying Heteroresistance in Populations ofSmall science · 2022Article
- Cyclic swelling enabled, electrically conductive 3D porous structures for microfluidic urinalysis devices.Extreme Mechanics Letters · 2022Article
- Combinatorial nanodroplet platform for screening antibiotic combinations.Lab on a chip · 2022Article
- Combating Antimicrobial Resistance via Single-Cell Diagnostic Technologies Powered by Droplet Microfluidics.Accounts of chemical research · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The lack of rapid antibiotic susceptibility tests adversely affects the treatment of bacterial infections and contributes to increased prevalence of multidrug-resistant bacteria. Here, we describe an all-electrical approach that allows for ultrasensitive measurement of growth signals from only tens of bacteria in a microfluidic device. Our device is essentially a set of microfluidic channels, each with a nanoconstriction at one end and cross-sectional dimensions close to that of a single bacterium. Flowing a liquid bacteria sample (e.g., urine) through the microchannels rapidly traps the bacteria in the device, allowing for subsequent incubation in drugs. We measure the electrical resistance of the microchannels, which increases (or decreases) in proportion to the number of bacteria in the microchannels. The method and device allow for rapid antibiotic susceptibility tests in about 2 h. Further, the short-time fluctuations in the electrical resistance during an antibiotic susceptibility test are correlated with the morphological changes of bacteria caused by the antibiotic. In contrast to other electrical approaches, the underlying geometric blockage effect provides a robust and sensitive signal, which is straightforward to interpret without electrical models. The approach also obviates the need for a high-resolution microscope and other complex equipment, making it potentially usable in resource-limited settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.