Evidence map›Paper›PMID 32348780›Full record

ArticleMolecular cell2020

A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.

Alexandra Lewis, Ahmet C Berkyurek, Andre Greiner, Ahilya N Sawh, Ajay Vashisht, Stephanie Merrett, Mathieu N Flamand, James Wohlschlegel, Mihail Sarov, Eric A Miska and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 4 countries.

Alexandra LewisDepartment of Biochemistry & Goodman Cancer Research Centre, McGill University, Montréal, QC H3A 1A3, Canada.
Ahmet C BerkyurekGurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.
Andre GreinerMolecular Cell Biology and Genetics, Max Planck Institute, 01307 Dresden, Germany.
Ahilya N SawhDepartment of Biochemistry & Goodman Cancer Research Centre, McGill University, Montréal, QC H3A 1A3, Canada.
Ajay VashishtDepartment of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Stephanie MerrettMolecular Cell Biology and Genetics, Max Planck Institute, 01307 Dresden, Germany.
Mathieu N FlamandDepartment of Biochemistry & Goodman Cancer Research Centre, McGill University, Montréal, QC H3A 1A3, Canada.
James WohlschlegelDepartment of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Mihail SarovMolecular Cell Biology and Genetics, Max Planck Institute, 01307 Dresden, Germany.
Eric A MiskaGurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.
Thomas F DuchaineDepartment of Biochemistry & Goodman Cancer Research Centre, McGill University, Montréal, QC H3A 1A3, Canada. Electronic address: thomas.duchaine@mcgill.ca.
Max Planck Institute of Molecular Cell Biology and Genetics · DEMcGill University · CAUniversity of California, Los Angeles · USUniversity of Cambridge · GBMcGill University Health Centre · CA

Funding

Cancer Research UK 18583Cancer Research UK 27826Cancer Research UK A14492Cancer Research UK A18583Wellcome Trust 092096Wellcome Trust 104640
6 · The paper itself

Abstract

Nuclear RNA interference (RNAi) pathways work together with histone modifications to regulate gene expression and enact an adaptive response to transposable RNA elements. In the germline, nuclear RNAi can lead to trans-generational epigenetic inheritance (TEI) of gene silencing. We identified and characterized a family of nuclear Argonaute-interacting proteins (ENRIs) that control the strength and target specificity of nuclear RNAi in C. elegans, ensuring faithful inheritance of epigenetic memories. ENRI-1/2 prevent misloading of the nuclear Argonaute NRDE-3 with small RNAs that normally effect maternal piRNAs, which prevents precocious nuclear translocation of NRDE-3 in the early embryo. Additionally, they are negative regulators of nuclear RNAi triggered from exogenous sources. Loss of ENRI-3, an unstable protein expressed mostly in the male germline, misdirects the RNAi response to transposable elements and impairs TEI. The ENRIs determine the potency and specificity of nuclear RNAi responses by gating small RNAs into specific nuclear Argonautes.

Indexed as

AnimalsArgonaute ProteinsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell NucleusGene SilencingGerm CellsNuclear ProteinsRNA-Binding ProteinsRNA, Double-StrandedRNA InterferenceRNA, NuclearRNA, Small InterferingArgonaute ProteinsCaenorhabditis elegans ProteinsNRDE-3 protein, C elegansNuclear ProteinsRNA-Binding ProteinsRNA, Double-StrandedRNA, NuclearRNA, Small Interfering22G-RNAArgonauteNRDE-3piRNARNAiRNA interferencetrans-generational epigenetic inheritance

Identifiers

PMID32348780
PMCPMC7613089
OpenAlexW3018428740

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.