ArticleeLife2020
Maternal spindle transfer overcomes embryo developmental arrest caused by ooplasmic defects in mice.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Molecular Drivers of Developmental Arrest in the Human Preimplantation Embryo: A Systematic Review and Critical Analysis Leading to Mapping Future Research.International journal of molecular sciences · 2021Pooled it
- Fertility clinics are offering young mitochondria in controversial fertility technique.Nature · 2026Article
- Current state of the art in the transmission of mitochondrial DNA pathogenic variants: reviewing preventive-therapeutic alternatives.Journal of assisted reproduction and genetics · 2026Review
- Mitochondrial replacement techniques for treating infertility.Journal of medical ethics · 2026Article
- Pronuclear Transfer and Maternal Spindle Transfer in the Mouse.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Mitochondrial dysfunction in oocytes: implications for fertility and ageing.Journal of ovarian research · 2025Review
- Nuclear transfer leads to aberrant cell cycle regulation and translation, as determined by gene expression, and selection of mitochondrial DNA in porcine blastocysts.NAR molecular medicine · 2024Article
- Article
- Mechanisms of mitochondrial dysfunction in ovarian aging and potential interventions.Frontiers in endocrinology · 2024Review
- Pronuclear transfer rescues poor embryo development ofHuman reproduction open · 2024Article
- Enucleated oocyte donation: first for infertility treatment, then for mitochondrial diseases.Journal of assisted reproduction and genetics · 2022Article
- Human germline nuclear transfer to overcome mitochondrial disease and failed fertilization after ICSI.Journal of assisted reproduction and genetics · 2022Article
- Germ Cell Derivation from Pluripotent Stem Cells for Understanding In Vitro Gametogenesis.Cells · 2021Review
- Prospects of Germline Nuclear Transfer in Women With Diminished Ovarian Reserve.Frontiers in endocrinology · 2021Review
- Mitochondrial Genetic Drift after Nuclear Transfer in Oocytes.International journal of molecular sciences · 2020Review
- Significance of Mitochondrial Dynamics in Reproductive Physiology: Current and Emerging Horizons in Mitochondrial Therapy for Assisted Reproductive Technologies.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
The developmental potential of early embryos is mainly dictated by the quality of the oocyte. Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte developmental competence. Our proof-of-concept experiments show that replacement of the entire cytoplasm of oocytes from a sensitive mouse strain overcomes massive embryo developmental arrest characteristic of non-manipulated oocytes. Genetic analysis confirmed minimal carryover of mtDNA following MST. Resulting mice showed low heteroplasmy levels in multiple organs at adult age, normal histology and fertility. Mice were followed for five generations (F5), revealing that heteroplasmy was reduced in F2 mice and was undetectable in the subsequent generations. This pre-clinical model demonstrates the high efficiency and potential of the MST technique, not only to prevent the transmission of mtDNA mutations, but also as a new potential treatment for patients with certain forms of infertility refractory to current clinical strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.