Evidence map›Paper›PMID 32347705›Full record

ArticleACS nano2020

Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires.

Yun X Chen, Nicole L Ing, Fengbin Wang, Dawei Xu, Nancy B Sloan, Nga T Lam, Daniel L Winter, Edward H Egelman, Allon I Hochbaum, Douglas S Clark and 1 more

Open access · greenAbstract read
In one paragraph

Article in ACS nano, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Yun X ChenSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Nicole L IngDepartment of Materials Science and Engineering, University of California, Irvine, California 92697, United States.
Fengbin WangDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, United States.
Dawei XuDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.
Nancy B SloanDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.
Nga T LamSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Daniel L WinterSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Edward H EgelmanDepartment of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, United States.
Allon I HochbaumDepartment of Materials Science and Engineering, University of California, Irvine, California 92697, United States.ORCID 0000-0002-5377-8065
Douglas S ClarkDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, California 94720, United States.ORCID 0000-0003-1516-035X
Dominic J GloverSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.ORCID 0000-0002-6819-4089
UNSW Sydney · AUUniversity of California, Berkeley · USUniversity of California, Irvine · USUniversity of Virginia · USLawrence Berkeley National Laboratory · US

Funding

Cryo-EM of Helical Protein and Nucleoprotein Polymers at Near Atomic ResolutionR35GM122510 · NIGMS · UNIVERSITY OF VIRGINIA · PI EDWARD H. EGELMAN · 2017 to 2026
$7.3M
NIGMS NIH HHS R35 GM122510
6 · The paper itself

Abstract

The transfer of electrons through protein complexes is central to cellular respiration. Exploiting proteins for charge transfer in a controllable fashion has the potential to revolutionize the integration of biological systems and electronic devices. Here we characterize the structure of an ultrastable protein filament and engineer the filament subunits to create electronically conductive nanowires under aqueous conditions. Cryoelectron microscopy was used to resolve the helical structure of gamma-prefoldin, a filamentous protein from a hyperthermophilic archaeon. Conjugation of tetra-heme c3-type cytochromes along the longitudinal axis of the filament created nanowires capable of long-range electron transfer. Electrochemical transport measurements indicated networks of the nanowires capable of conducting current between electrodes at the redox potential of the cytochromes. Functionalization of these highly engineerable nanowires with other molecules, such as redox enzymes, may be useful for bioelectronic applications.

Indexed as

MetalloproteinsNanowiresCryoelectron MicroscopyElectric ConductivityElectron TransportMetalloproteinsconductive biomaterialselectron transportnanowiresprotein engineeringprotein self-assembly

Identifiers

PMID32347705
PMCPMC8034818
OpenAlexW3020776358

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.