Evidence map›Paper›PMID 32347454›Full record

ArticleTissue engineering and regenerative medicine2020

Osteoblast-Based Therapy-A New Approach for Bone Repair in Osteoporosis: Pre-Clinical Setting.

Nadia Samy Mahmoud, Mohamed Ragaa Mohamed, Mohamed Ahmed Mohamed Ali, Hadeer Ahmed Aglan, Khalda Sayed Amr, Hanaa Hamdy Ahmed

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Nadia Samy MahmoudHormones Department, Medical Research Division, National Research Centre, 33 EL Bohouth St. (former EL -Tahrir st.)-Dokki, Giza, 12622, Egypt. nadiasamy@sci.asu.edu.eg.ORCID http://orcid.org/0000-0002-9085-4063
Mohamed Ragaa MohamedBiochemistry Department, Faculty of Science, Ain Shams University, El-Khalyfa El-Ma'moun St., Abbasya, Cairo, 11566, Egypt.
Mohamed Ahmed Mohamed AliBiochemistry Department, Faculty of Science, Ain Shams University, El-Khalyfa El-Ma'moun St., Abbasya, Cairo, 11566, Egypt.
Hadeer Ahmed AglanHormones Department, Medical Research Division, National Research Centre, 33 EL Bohouth St. (former EL -Tahrir st.)-Dokki, Giza, 12622, Egypt.
Khalda Sayed AmrMedical Molecular Genetics Department, Human Genetics and Genome Researches Division, National Research Centre, 33 EL Bohouth St. (former EL -Tahrir St.)-Dokki, Giza, 12622, Egypt.
Hanaa Hamdy AhmedHormones Department, Medical Research Division, National Research Centre, 33 EL Bohouth St. (former EL -Tahrir st.)-Dokki, Giza, 12622, Egypt.
National Research Centre · EGAin Shams University · EG

Funding

National Research Centre, Egypt Thesis fund No. 71511
6 · The paper itself

Abstract

backgroundOsteoporosis is a metabolic bone disease characterized by low bone density resulting in increased fracture susceptibility. This research was constructed to uncover the potential therapeutic application of osteoblasts transplantation, generated upon culturing male rat bone marrow-derived mesenchymal stem cells (BM-MSCs) in osteogenic medium (OM), OM containing gold (Au-NPs) or gold/hydroxyapatite (Au/HA-NPs) nanoparticles, in ovariectomized rats to counteract osteoporosis.

methodsForty rats were randomized into: (1) negative control, (2) osteoporotic rats, whereas groups (3), (4) and (5) constituted osteoporotic rats treated with osteoblasts yielded from culturing BM-MSCs in OM, OM plus Au-NPs or Au/HA-NPs, respectively. After 3 months, osterix (OSX), bone alkaline phosphatase (BALP), sclerostin (SOST) and bone sialoprotein (BSP) serum levels were assessed. In addition, gene expression levels of cathepsin K, receptor activator of nuclear factor-κb ligand (RANKL), osteoprotegerin (OPG) and RANKL/OPG ratio were evaluated using real-time PCR. Moreover, histological investigation of femur bone tissues in different groups was performed. The homing of implanted osteoblasts to the osteoporotic femur bone of rats was documented by Sex determining region Y gene detection in bone tissue.

resultsOur results indicated that osteoblasts infusion significantly blunted serum BALP, BSP and SOST levels, while significantly elevated OSX level. Also, they brought about significant down-regulation in gene expression levels of cathepsin K, RANKL and RANKL/OPG ratio versus untreated osteoporotic rats. Additionally, osteoblasts nidation could restore bone histoarchitecture.

conclusionThese findings offer scientific evidence that transplanting osteoblasts in osteoporotic rats regains the homeostasis of the bone remodeling cycle, thus providing a promising treatment strategy for primary osteoporosis.

Indexed as

OsteogenesisAlkaline PhosphataseAnimalsBone RemodelingCathepsin KDurapatiteFemurGene ExpressionMaleMesenchymal Stem CellsOsteoblastsOsteoporosisOsteoprotegerinRANK LigandRatsAlkaline PhosphataseCathepsin KDurapatiteOsteoprotegerinRANK LigandTNFSF11 protein, humanBone remodelingGoldHydroxyapatiteOsteoblastsOsteoporosis

Identifiers

PMID32347454
PMCPMC7260339
OpenAlexW3020197126

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.