Evidence map›Paper›PMID 32346060›Full record

ArticleNature communications2020

Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.

Sebastian Karpf, Carson T Riche, Dino Di Carlo, Anubhuti Goel, William A Zeiger, Anand Suresh, Carlos Portera-Cailliau, Bahram Jalali

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023
    Review
  17. Article
  18. Article
  19. Fourier Domain Mode Locked Laser and Its Applications.Sensors (Basel, Switzerland) · 2022
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sebastian KarpfDepartment of Electrical Engineering and Computational Science, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA. sebastian.karpf@uni-luebeck.de.ORCID http://orcid.org/0000-0002-7665-5914
Carson T RicheDepartment of Bioengineering, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.
Dino Di CarloDepartment of Bioengineering, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.
Anubhuti GoelDepartment of Neurology, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.
William A ZeigerDepartment of Neurology, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.
Anand SureshDepartment of Neurology, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.
Carlos Portera-CailliauDepartment of Neurology, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.ORCID http://orcid.org/0000-0001-5735-6380
Bahram JalaliDepartment of Electrical Engineering and Computational Science, University of California, Los Angeles (UCLA), Los Angeles, CA-90095, USA.

Funding

Imaging the origin of dendritic spine abnormalities in fragile X miceR01HD054453 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Carlos Portera-Cailliau · 2007 to 2026
$6.5M
Kilohertz frame rate two-photon and confocal fluorescence microscope enabled by rR21GM107924 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JALALI, BAHRAM · 2014 to 2016
$548k
A Frequency-multiplexed flow cytometer for high throughput screening and drug discoveryR21EB019645 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DI CARLO, DINO, JALALI, BAHRAM · 2015 to 2016
$424k
NIBIB NIH HHS R21 EB019645NICHD NIH HHS R01 HD054453NIGMS NIH HHS R21 GM107924
6 · The paper itself

Abstract

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting.

Indexed as

Microscopy, Fluorescence, MultiphotonEuglenaImaging, Three-DimensionalTime Factors

Identifiers

PMID32346060
PMCPMC7188897

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.