Evidence map›Paper›PMID 32345643›Full record

ArticlemBio2020

Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System.

Liqiong Liu, Susu Jiang, Mai Xing, Chao Chen, Chongde Lai, Na Li, Guangfeng Liu, Dan Wu, Haiyan Gao, Liang Hong and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Sulfur incorporation into biomolecules: recent advances.Critical reviews in biochemistry and molecular biology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 1 country.

Liqiong Liu *State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, China.
Susu Jiang *Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Mai XingKey Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Chao ChenTaihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Chongde LaiJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Na LiNational Facility for Protein Science Shanghai, Zhangjiang Lab, Shanghai, China.
Guangfeng LiuNational Facility for Protein Science Shanghai, Zhangjiang Lab, Shanghai, China.
Dan WuKey Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Haiyan GaoKey Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Liang HongSchool of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai, China.
Pan TanSchool of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai, China.
Shi ChenTaihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Zixin DengState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, China.
Geng WuState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai, China geng.wu@sjtu.edu.cn lianrong@whu.edu.cn.
Lianrong WangTaihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China geng.wu@sjtu.edu.cn lianrong@whu.edu.cn.
Shanghai Jiao Tong University · CNWuhan University · CNZhongnan Hospital of Wuhan University · CNHubei University of Medicine · CNJiangxi Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA phosphorothioate (PT) modification, in which the nonbridging oxygen in the sugar-phosphate backbone is substituted by sulfur, is catalyzed by DndABCDE or SspABCD in a double-stranded or single-stranded manner, respectively. In Dnd and Ssp systems, mobilization of sulfur in PT formation starts with the activation of the sulfur atom of cysteine catalyzed by the DndA and SspA cysteine desulfurases, respectively. Despite playing the same biochemical role, SspA cannot be functionally replaced by DndA, indicating its unique physiological properties. In this study, we solved the crystal structure of

Indexed as

Bacterial ProteinsCarbon-Sulfur LyasesCatalytic DomainCrystallography, X-RayCysteineDNAIron-Sulfur ProteinsMolecular Dynamics SimulationPhosphorothioate OligonucleotidesPyridoxal PhosphateSulfurVibrioBacterial ProteinsCarbon-Sulfur LyasesCysteinecysteine desulfuraseDNAIron-Sulfur ProteinsPhosphorothioate OligonucleotidesPyridoxal PhosphateSulfurcrystal structurecysteine desulfuraseDNA PT modificationSsp system

Identifiers

PMID32345643
PMCPMC7188994
OpenAlexW3018241995

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.