ArticleMicrobial cell factories2020
Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Can Mammalian Reproductive Health Withstand Massive Exposure to Polystyrene Micro- and Nanoplastic Derivatives? A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Plastic-algae interactions in freshwater and marine systems: mechanisms, evidence and prospects for bioremediation.World journal of microbiology & biotechnology · 2026Review
- Rethinking microplastic cleanup: sustainable bioremediation compared to conventional physical-chemical methods.RSC advances · 2026Review
- Plastic-Degrading Microorganisms: Biodegradation Pathways and Habitat Origins.Molecules (Basel, Switzerland) · 2026Review
- Genetically engineered organisms for sustainable bioremediation of polluted environments.Biodegradation · 2026Review
- Microbial mechanisms as tools for monitoring and treating emerging contaminants in urban pollution: an overview.Archives of microbiology · 2026Review
- Proteomic characterization ofFrontiers in microbiology · 2026Article
- Microbial plastic degradation: enzymes, pathways, challenges, and perspectives.Microbiology and molecular biology reviews : MMBR · 2025Review
- Construction of microbial systems for polyethylene terephthalate degradation.Synthetic and systems biotechnology · 2025Review
- Degradation of polyethylene terephthalate by microorganisms and their enzymes: A review of experimental and in silico research.World journal of microbiology & biotechnology · 2025Review
- EngineeringInternational journal of molecular sciences · 2025Review
- Biosphere Plastic Contamination and Microbial Alternatives for a Sustainable Degradation of Plastic Waste.Microorganisms · 2025Review
- Responses of soil biochemical properties andFrontiers in plant science · 2025Article
- In silico structural analysis and biochemical characterization of a novel PETase from AntarcticComputational and structural biotechnology journal · 2025Article
- Efficient biodegradation and upcycling of polyethylene terephthalate mediated by cell-factories.Frontiers in microbiology · 2025Review
- Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation.ACS synthetic biology · 2024Review
- Harnessing photosynthetic microorganisms for enhanced bioremediation of microplastics: A comprehensive review.Environmental science and ecotechnology · 2024Review
- Microorganism-mediated biodegradation for effective management and/or removal of micro-plastics from the environment: a comprehensive review.Archives of microbiology · 2024Review
- Recent advances in microbial and enzymatic engineering for the biodegradation of micro- and nanoplastics.RSC advances · 2024Review
- Insight on recently discovered PET polyester-degrading enzymes, thermostability and activity analyses.3 Biotech · 2024Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundFor decades, plastic has been a valuable global product due to its convenience and low price. For example, polyethylene terephthalate (PET) was one of the most popular materials for disposable bottles due to its beneficial properties, namely impact resistance, high clarity, and light weight. Increasing demand of plastic resulted in indiscriminate disposal by consumers, causing severe accumulation of plastic wastes. Because of this, scientists have made great efforts to find a way to biologically treat plastic wastes. As a result, a novel plastic degradation enzyme, PETase, which can hydrolyze PET, was discovered in Ideonella sakaiensis 201-F6 in 2016.
resultsA green algae, Chlamydomonas reinhardtii, which produces PETase, was developed for this study. Two representative strains (C. reinhardtii CC-124 and CC-503) were examined, and we found that CC-124 could express PETase well. To verify the catalytic activity of PETase produced by C. reinhardtii, cell lysate of the transformant and PET samples were co-incubated at 30 °C for up to 4 weeks. After incubation, terephthalic acid (TPA), i.e. the fully-degraded form of PET, was detected by high performance liquid chromatography analysis. Additionally, morphological changes, such as holes and dents on the surface of PET film, were observed using scanning electron microscopy.
conclusionsA PET hydrolyzing enzyme, PETase, was successfully expressed in C. reinhardtii, and its catalytic activity was demonstrated. To the best of our knowledge, this is the first case of PETase expression in green algae.
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Registered trials
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