Evidence map›Paper›PMID 32344594›Full record

ArticleInternational journal of molecular sciences2020

FGCaMP7, an Improved Version of Fungi-Based Ratiometric Calcium Indicator for In Vivo Visualization of Neuronal Activity.

Natalia V Barykina, Vladimir P Sotskov, Anna M Gruzdeva, You Kure Wu, Ruben Portugues, Oksana M Subach, Elizaveta S Chefanova, Viktor V Plusnin, Olga I Ivashkina, Konstantin V Anokhin and 8 more

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 26 citations in OpenAlex.

  1. Split genetically encoded calcium indicators for interorganellar junctions.Proceedings of the National Academy of Sciences of the United States of America · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 8 institutions in 2 countries.

Natalia V BarykinaLaboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology, 125315 Moscow, Russia.ORCID 0000-0001-5835-8388
Vladimir P SotskovInstitute for Advanced Brain Studies, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.ORCID 0000-0002-1729-5744
Anna M GruzdevaInstitute for Advanced Brain Studies, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.ORCID 0000-0001-5691-9057
You Kure WuSensorimotor Control Research Group, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
Ruben PortuguesSensorimotor Control Research Group, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
Oksana M SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Elizaveta S ChefanovaDepartment of NBIC-technologies, Moscow Institute of Physics and Technology, 123182 Moscow, Russia.
Viktor V PlusninComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.ORCID 0000-0001-9784-8283
Olga I IvashkinaLaboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology, 125315 Moscow, Russia.
Konstantin V AnokhinLaboratory for Neurobiology of Memory, P.K. Anokhin Research Institute of Normal Physiology, 125315 Moscow, Russia.
Anna V VlaskinaComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Dmitry A KorzhenevskiyComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Alena Y NikolaevaComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
Konstantin M BoykoBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.ORCID 0000-0001-8229-189X
Tatiana V RakitinaComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.ORCID 0000-0002-3096-8659
Anna M VarizhukDepartment of Biophysics, Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.
Galina E PozmogovaDepartment of Biophysics, Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia.
Fedor V SubachComplex of NBICS Technologies, National Research Center "Kurchatov Institute", 123182 Moscow, Russia.ORCID 0000-0003-2720-7821
Kurchatov Institute · RULomonosov Moscow State University · RUMoscow Institute of Physics and Technology · RUResearch Centre for Medical Genetics · RUA N Bach Institute of Biochemistry · RUInstitute of Normal Physiology named after P.K. Anokhin · RUMax Planck Institute of Neurobiology · DEMunich Cluster for Systems Neurology · DE

Funding

Deutsche Forschungsgemeinschaft EXC 2145 SyNergy - ID 390857198internal grant of the National Research Center Kurchatov Institute №1360 of 25.06.2019Ministry of Science and Higher Education of the Russian Federation to K.M.B.Russian Foundation for Basic Research 17-00-00215Russian Foundation for Basic Research 17-29-07083Russian Foundation for Basic Research 18-34-00640Russian Foundation for Basic Research 19-04-00395Russian Foundation for Basic Research 19-315-80020Russian Foundation for Basic Research 19-34-70004Russian Foundation for Basic Research 20-015-00427Russian Science Foundation 16-15-10323the Ministry of Science and Higher Education of the Russian Federation 075-15-2019-1669
6 · The paper itself

Abstract

Genetically encoded calcium indicators (GECIs) have become a widespread tool for the visualization of neuronal activity. As compared to popular GCaMP GECIs, the FGCaMP indicator benefits from calmodulin and M13-peptide from the fungi

Indexed as

Gene ExpressionMolecular ImagingAction PotentialsAnimalsCalciumCalcium-Binding ProteinsFungiGenes, ReporterHeLa CellsHumansMiceMicroscopy, FluorescenceModels, MolecularNeuronsProtein ConformationProtein EngineeringCalciumCalcium-Binding Proteinscalcium imagingcrystal structureFGCaMPFGCaMP7genetically encoded calcium indicatorprotein engineering

Identifiers

PMID32344594
PMCPMC7215472
OpenAlexW3018745561

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.