ArticleMicrobial biotechnology2021
Thermophilic whole-cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed, 1 synthesis or guideline pooled it, 209 citations in OpenAlex.
- The diversity of PET degrading enzymes: A systematic review of sequence, structure, and function.Protein science : a publication of the Protein Society · 2025Pooled it
- Characterization of novel phage Henuyfy11N: a potential therapeutic agent against extended-spectrum β-lactamase (ESBL)-producingMicrobiology spectrum · 2026Article
- Sustainable and High-Performance Food-Packaging Films from Poly(butylene 2,5-furanoate) and Poly(pentamethylene 2,5-furanoate) Blends.Polymers · 2026Article
- Fiber to fragment: a review of microplastics from textile industry effluents, their detection, ecological impact, and sustainable microbial biodegradation strategies.Archives of microbiology · 2026Review
- Reflections on bio-based PET and plastic waste management: a responsible research and innovation approach.Nature communications · 2026Review
- Microplastic Pollution: Advancements in Mitigation, Policy Challenges, and Future Directions.Current microbiology · 2026Review
- Microbial mechanisms as tools for monitoring and treating emerging contaminants in urban pollution: an overview.Archives of microbiology · 2026Review
- Article
- Microbial plastic degradation: enzymes, pathways, challenges, and perspectives.Microbiology and molecular biology reviews : MMBR · 2025Review
- Construction of microbial systems for polyethylene terephthalate degradation.Synthetic and systems biotechnology · 2025Review
- Insights into Microbial Enzymatic Biodegradation of Plastics and Microplastics: Technological Updates.ACS environmental Au · 2025Review
- Discovering rare thermophilic Parageobacillus caldoxylosilyticus from hot spring with early plastic-degrading potential revealed from bioassays and gene annotation studies.Folia microbiologica · 2025Article
- Standardization guidelines and future trends for PET hydrolase research.Nature communications · 2025Review
- Improving the thermostability and substrate affinity of3 Biotech · 2025Article
- Emerging Biochemical Conversion for Plastic Waste Management: A Review.Molecules (Basel, Switzerland) · 2025Review
- Thermostability and Activity Improvements of PETase fromACS omega · 2025Article
- Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes.Microbial cell factories · 2024Article
- Review
- Degradation of polyethylene terephthalate (PET) plastics by wastewater bacteria engineered via conjugation.Microbial biotechnology · 2024Article
- Fully Bio-Based Blends of Poly (Pentamethylene Furanoate) and Poly (Hexamethylene Furanoate) for Sustainable and Flexible Packaging.Polymers · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyethylene terephthalate (PET) is a mass-produced synthetic polyester contributing remarkably to the accumulation of solid plastics waste and plastics pollution in the natural environments. Recently, bioremediation of plastics waste using engineered enzymes has emerged as an eco-friendly alternative approach for the future plastic circular economy. Here we genetically engineered a thermophilic anaerobic bacterium, Clostridium thermocellum, to enable the secretory expression of a thermophilic cutinase (LCC), which was originally isolated from a plant compost metagenome and can degrade PET at up to 70°C. This engineered whole-cell biocatalyst allowed a simultaneous high-level expression of LCC and conspicuous degradation of commercial PET films at 60°C. After 14 days incubation of a batch culture, more than 60% of the initial mass of a PET film (approximately 50 mg) was converted into soluble monomer feedstocks, indicating a markedly higher degradation performance than previously reported whole-cell-based PET biodegradation systems using mesophilic bacteria or microalgae. Our findings provide clear evidence that, compared to mesophilic species, thermophilic microbes are a more promising synthetic microbial chassis for developing future biodegradation processes of PET waste.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.