ArticleMicrobiologyOpen2020
Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.
Article in MicrobiologyOpen, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Division of labour enables Yarrowia lipolytica synthetic consortia to achieve higher growth rates from starch.Microbial cell factories · 2026Review
- Advances in microbial biofuel production by metabolic and enzyme engineering, synthetic biology, metagenomics, and genome editing applications.Emerging topics in life sciences · 2025Review
- Review
- Review
- Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast.Journal of biological engineering · 2024Review
- Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts.Synthetic and systems biotechnology · 2023Review
- Survey of nonconventional yeasts for lipid and hydrocarbon biotechnology.Journal of industrial microbiology & biotechnology · 2022Article
- Microbial production of butyl butyrate: from single strain to cognate consortium.Bioresources and bioprocessing · 2021Review
- Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters.Biotechnology for biofuels · 2021Article
- The Studies in Constructing Yeast Cell Factories for the Production of Fatty Acid Alkyl Esters.Frontiers in bioengineering and biotechnology · 2021Review
- Sustainable production of FAEE biodiesel using the oleaginous yeast Yarrowia lipolytica.MicrobiologyOpen · 2020Article
- Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fatty acid ethyl esters (FAEEs) are fatty acid-derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid-derived products due to its excellent lipid accumulation capacity. In this proof-of-principle study, several metabolic engineering strategies were applied for the overproduction of FAEE biodiesel in Y. lipolytica. Here, chromosome-based co-overexpression of two heterologous genes, namely, PDC1 (encoding pyruvate decarboxylase) and ADH1 (encoding alcohol dehydrogenase) from Saccharomyces cerevisiae, and the endogenous GAPDH (encoding glyceraldehyde-3-phosphate dehydrogenase) gene of Y. lipolytica resulted in successful biosynthesis of ethanol at 70.8 mg/L in Y. lipolytica. The engineered Y. lipolytica strain expressing the ethanol synthetic pathway together with a heterologous wax ester synthase (MhWS) exhibited the highest FAEE titer of 360.8 mg/L, which is 3.8-fold higher than that of the control strain when 2% exogenous ethanol was added to the culture medium of Y. lipolytica. Furthermore, a synthetic microbial consortium comprising an engineered Y. lipolytica strain that heterologously expressed MhWS and a S. cerevisiae strain that could provide ethanol as a substrate for the production of the final product in the final engineered Y. lipolytica strain was created in this study. Finally, this synthetic consortium produced FAEE biodiesel at a titer of 4.8 mg/L under the optimum coculture conditions.
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